One in Denmark, one in Italy, one in France/Singapore and in Germany!
The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
The Live Cell imaging facility will run again its intensive light microscopy course in Jan-Feb 2021.
The schedule can be found here.
As usual, all lectures will be publicly broadcasted live. So if you think that a lecture could be useful for you, you are welcome to listen, without registration, by following this link.
Due to the current pandemics, only registered students will be allowed in the lecture room.
The course comprises lectures, workshops, imaging of your own sample, demos… It will run 26 Jan-12 Feb, 3 days/week (tues-thurs) 9:00-17:15.
There are only 16 spots. The course counts for 6 credit points.
The rest of the time (the course counts for 4 weeks) is used for home assignments. Mondays and Fridays will also be used for individual workshops where we image your own sample.
The application process is open now until the 16th of November. Please read carefully the eligibility criteria. You will need to sent me some images of your sample at the time of application. This course is only open to people who already have some experience of fluorescence microscopy.
Microscopy: improve your imaging skills – from sample preparation to image analysis
At the end of the course, the participants will be able to:
1- Describe the difference between wide field, confocal and light sheet microscopes as well as the different types of confocal microscopes and choose which system is most suited to their experiments
2- Pick the best combination of fluorophores for their experiment by matching their spectra with the microscope light source and filters, identify and eliminate bleed-through and cross-excitation problems
3- Explain objective specifications and limitations and choose the appropriate objective for their own experiments
4- Describe how to fix, mount and handle their sample in a way that is optimal for imaging
5- Find their sample and the area of interest without bleaching it
6- Adjust the condenser for proper DIC imaging (Koehlering)
7- Explain how to set the following parameters on a wide field, a confocal or a light sheet system to best match the requirements of their sample and reliably answer their scientific question: resolution, pixel size, averaging, scan speed, illumination power, detector gain and offset, camera readout rate, exposure time and camera binning
8- Explain which applications require a hardware or a software autofocus, a spectral detector, a resonance scanner, two-photon or super resolution microscopy
9- Explain the advantages in using the automation of a microscope system to collect multidimensional data
10- Explain how to deal with images before publication in scientific journals
11- Describe the imaging requirements for automated image analysis
12- Run an image analysis pipeline on freeware (ImageJ/FIJI, Cell Profiler) designed for their own images and scientific question.
Please spread the word to your colleagues.
We are looking forward to meeting you and your sample! 😃
Our dear Tweety microscope, which was simplest and cutest of all the LCI systems, has muted into our most sophisticated power machine!
On the 24th at 10, we will run an online demo (link below) to show what our upgraded Tweety can deliver:
After the demo, the LCI users who have already been trained on our widefield systems can get access to Tweety for free after a mandatory short training.
Please add the demo in your calendar and make sure to test the link ahead of the meeting.
Link to the Zoom Meeting on the 24th at 10am: https://ki-se.zoom.us/j/7302561100
– Image.sc and microlist
Post your microscopy, sample preparation and image analysis questions
– Confocal server
Old email-based forum that is followed by tons of people across the globe as well as most microscopy companies. Post your microscopy and sample preparation questions.
– Image J/Fiji
Great freeware to open images acquired on any microscope, resize, crop, change contrast, create movies, annotate, analyze and much more.
– Cell Profiler
Freeware for automated image analysis including machine learning.
– KNIME
Open source to build a custom made image analysis pipeline
– Icy
Great freeware to open images acquired on any microscope, resize, crop, change contrast, create movies, annotate, analyze and much more.
Light microscopy jobs, meetings, courses, networking… in Europe. Remember to mail them that you want to show up on their map of European microscopy!
Loads of information from which companies/institute works with research to how to find an apartment when moving to another lab, find work, know your rights, express your opinion about researchers’ work conditions…
– Kurt Thorn’s excellent microscopy blog
I highly recommend this blog even if it is not updated anymore. Monthly digest of major microscopy related articles. Loads of tips.
– Microscopy Education by Nikon
– Microscopy Education by Olympus
– Microscopy Education by ZEISS
Here is a nice opportunity to change career if you feel like it and move to admin while keeping a strong microscopy feel.
Have a look at here.
🙂
CLIJ2 allows you to use ImageJ/Fiji on GPU instead if CPU processing, so much faster! 🙂
Here is a nice article about what CLIJ2 can do. By the way, this article is published on a new imaging forum called FocalPlane. Check it out! And here is the presentation of how to use CLIJ2 at one of the recent Neubias event in May 2020.
If you have an analysis pipeline built in Fiji, Icy or Matlab and processing takes a long time, CLIJ2 will help you a lot.
Researcher/ Light Microscopy Specialist (m/f/d)
UKE Light Microscopy Facility
Pay grade 13 TV-KAH
Work with your finger on the pulse in a compressed cosmos that is forever changing. A work environment where a person can make a difference because they have the space and environment to implement change and think differently.
*We offer*
This position is initially limited for two years part as part of a project. An extension of this period is envisioned. This is a full-time position (38.5 h/week), which can also be filled with part-time models.
*What is expected of you*
*What we look forward to
Contact person: Dr. Virgilio Failla Tel. +49 (0)40 7410 – 51958/ E-Mail: a.failla@uke.de
We offer a work environment that offers everyone the same chances, regardless of age, sex, sexual preference, disability, ethnic background or religion. We are actively trying to increase the number of women in senior roles, especially in research and teaching. In the event of equal levels of qualification, preference will be given to female candidates.
The same rule will apply to all genders when one gender is deemed to be under-represented in the department relevant to the available position.
Severely disabled applicant with essentially identical technical and personal suitability will be preferentially selected.
We look forward to receiving your full application by the reference code
2020-321 until 29.6.2020.
—
Dr. Antonio Virgilio Failla
Head of Light microscopy facility
Universitätsklinikum Hamburg-Eppendorf
UKE Microscopy Imaging Facility (UMIF)
Campus Forschung (N27), EG, Room 00.108.2 Martinistraße 52
20246 Hamburg
Tel.: (+49) 40-7410-51985
Mobil: (+49) (0)152-22815841
A facility manager job in the sun of Barcelona! 🙂
Check out this webinar tomorrow by Rickardo Henriques, the inventor of SRRF (surf), post processing magic for all types of images!
*Title:* Open and accessible cutting-edge technology for super-resolution and machine-learning enabled microscopy
*When: *June 5th 4:00 pm CEST
*How to access/register*
And here’s a small teaser if you want to quickly see the topics we’ll cover.