Congratulations to our dear in-house image analyst Gisele Miranda who got the prestigious Chan Zuckerberg Initiative grant in December! 🙂
Gisele got this grant thanks to the fruitful collaboration between the BioImage Informatics facility at Scilife and the Live Cell Imaging facility at KI. We are delighted for her and for all the LCI users as this will allow us to keep working with Gisele for many years.
Congratulations Gisele! Very well-deserved! 🙂
Look at what CZI has chosen as their symbol of Science: a microscope!
Nov 30th-Dec 2nd, Janelia Research Center organizes a great image analysis workshop with loads of goodies. Everything is online and free. There are in depth and more general workshops about Deep Learning with different freeware, programming and scripting of all sorts, Big data things… Check the program here.
Our dear Tweety microscope, which was simplest and cutest of all the LCI systems, has muted into our most sophisticated power machine!
On the 24th at 10, we will run an online demo (link below) to show what our upgraded Tweety can deliver:
- Much larger field of view (from 18 mm diagonal to 25 mm)
- Upgraded single point confocal on the left side
- Resonant scanner with 1024×1024 pixels (compared to 512×512 on our other resonant scanners), still the same speed (30 fps) and improved low noise
- Spinning disk confocal on the right side with bypass to image wide field
- 2 very sensitive cameras on the right side: one with the very large field of view and 11um pixels for best sensitivity and one with the normal field of view and 6.45 pixels for best resolution
- Our great Primo is still on the back of the microscope to allow micropatterning of proteins at the bottom of a dish or micromanufacturing of wells in the shape/pattern of your choice
- 2 wonderful silicon immersion objectives specialized for tissue imaging with automatic correction ring: 20x/1.05 and 40x/1.25
After the demo, the LCI users who have already been trained on our widefield systems can get access to Tweety for free after a mandatory short training.
Please add the demo in your calendar and make sure to test the link ahead of the meeting.
Link to the Zoom Meeting on the 24th at 10am: https://ki-se.zoom.us/j/7302561100
Updates on the next events of the NEUBIAS Academy@Home Webinar series,
Newly confirmed events:
5 May: ilastik beyond pixel classification, by Anna Kreshuk and Dominik Kutra-
6 May: GPU-Accelerated Image Processing with CLIJ2, by Robert Haase
7 May: Interactive Bioimage Analysis with Python and Jupyter, by Guillaume Witz
Upcoming events open to registration:
LAST CHANCE TO REGISTER:
28 April: Introduction to nuclei segmentation with StarDist, by Martin Weigert et al
29 April: Quantitative Pathology and Bioimage Analysis: QuPath v0.2.0, By Pete Bankhead
30 April: Advanced Image Processing with MorphoLibJ, by David Legland
Two weeks after the opening of the Academy and of the registrations, Webinars and online courses have already attracted over 5,000 registrations!
The events are recorded and some are already available on the Youtube NEUBIAS Channel.
Furthermore, a thread will be opened in the image.sc Forum to report Q&As and to welcome further questions/comments for each event.
You’ll find more information here.
In these strange Coronavirus time, one tends to sit at home in front of one’s computer. Following the Corona pandemics, it is not hard to predict a back ache pandemic!
I have been using Workrave for years and it has cured by shoulder ache! 🙂 Workrave is a freeware that can be downloaded from here.
Basically it locks your keyboard and mouse at the interval of time you decide and for how long as you decide. You can even set a maximum work time per day, a longer interruption for lunch or follow some simple stretching programs during the interruption.
I have simply set mine to lock my computer for 2 min every 30 min. Works a charm. 🙂
Neubias is back with new ideas! Neubias is the Network of European Bioimage Analysts and what they burn for is to help scientists analyze their images.
Possibly inspired by the Corona time, they will start an online school for image analysis based on video tutorials and online events.
Have a look at their new page called Neubias academy where they announce several events coming up in the next few months.
Ever wondered if that antibody you used throughout your whole PhD was actually also binding to something else than its supposed target protein?
Antibody validation in tissue staining is a very difficult task!
Here is a great step-by-step validation protocol published by EuroMabNet, a network of scientists who try to improve antibody validation.
And this paper gives a useful flow chart for antibody validation.
And here is the 5 pillars of antibody validation paper which explains what can be done to validate antibodies.
Imagine starting a study about some cool protein.
You find some useful articles on Google Scholar. In one paper, an antibody is mentioned. The name of the company that sold it to the authors is mentioned in the Material and Method but unfortunately that company has closed down or has been swallowed by one of the Pharma giants so you cannot order. Then you realize that the company was not producing any antibodies anyway, they were buying it from another company so there is no way to trace and buy the same antibody. Nightmare…
Then imagine that instead, the paper mentions the RRid number for that antibody. You do not know about what that is but you check and find this paper that explains it all.
Now suddenly, not only you can find on the Scicrunch website which company produces this antibody and which resells it so you can buy it, but you can also search pubmed for the RRid and find all the articles that mention it, opening your eyes to lots of results about your protein that have been published specifically with using that antibody. Now you can also check if the antibody gives consistent results!
And imagine being to do this for your favorite mouse model as well. See all publications that have mentioned your mouse RRId!
But it relies on you writing the RRid of your antibody or mouse in your next publication so think about it! 😀
Zell Kontakt is a German company making fantastic glass bottom dishes and plate. Check out why the LCI facility likes their products (absolutely no commercial interests!).
Great products but crap communication skills! Either their website is down or they do not answer the phone and emails and anyway if they do, make sure you have a German colleague around because no one speaks English.
Now we have found a way around the problem: MoBiTec sells Zell Kontakt products. 🙂
If you are at KI and need to find a KI-certified supplier, just come and ask us. 🙂