Cool service – The Live Cell Imaging facility

Free, online microscopy course by the LCI core facility

Dear all

Kindly forward to anyone who might be interested.

It is my pleasure to invite you to follow our free (no registration) online microscopy course.

The course schedule and Zoom link are found our course webpage.

The public program (everything in blue in the course schedule) starts next week on Thursday 22^th of Jan and runs until the 13^th of February.

Here is a selection of what we will talk about:

Optics and image formation,
How microscopes work
Objectives and refraction index
Cameras and detectors, Noise and background, Bit depth and saturation
Sample preparation, Immunostaining, Clearing
Resolution and contrast, Nyquist sampling, how to choose microscope settings
Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
Image processing and analysis

All lectures will be broadcasted live on Zoom, recorded then posted the same day on our Youtube channel.

Here you can read the Learning Objectives of the course.

Do not miss the kind testimonies on our course webpage! 🙂

The LCI core facility team, Stockholm, Sweden

Jobs opening at QUAREP-LIMI

A rare opportunity for those interested in contributing to Quality and Reproducibility within Light Microscopy (Quarep-LiMi consortium).

The QUAREP-LiMi network is currently recruiting for three positions related to Light Microscopy Metadata, Quality Control, and Community Management to work within the QUAREP-LiMi initiative (quarep.org):

- Lead Specialist – Light Microscopy Model (LiMi-Model)
- Lead Specialist – Validation Team
- Community Coordinator – QUAREP-LiMi

You can find more details about the positions here.

Please send your application, including supporting documents (CV, motivation letter, references, Certificates), and anything else that might help our decision, by 15 January 2026 at the latest, using this application form.

Useful tips on the Nikon E-learning platform

Dear all

Have a quick look at Nikon’s e-learning platform! There are many useful tips and reminders of what we teach during the LCI training and course. 🙂

For example, here is a reminder of how refractive index mismatch distorts your images and when you should be concerned. 🙂

High Speed Multiphoton Imaging of Dynamic Processes In Vivo at Stockholm University

Intravital people might be interested in this event at the Intravital Microscopy Stockholm University facility:

The online LCI microscopy course: Microscopy: improve your imaging skills – from sample preparation to image analysis.

Dear all

It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28^th of Jan) and runs until the 13^th of February.

As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

Here is a selection of what we will talk about:

Optics and image formation,
How microscopes work
Objectives and refraction index
Cameras and detectors, Noise and background, Bit depth and saturation
Sample preparation, Immunostaining, Clearing
Resolution and contrast, Nyquist sampling, how to choose microscope settings
Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
Image processing and analysis

Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.

Scroll down to read the kind testimonies of our dear students! 🙂

We hope that you will enjoy the LCI facility online microscopy course!

Kindly forward to anyone who might be interested.

The LCI team

Direct label of primary antibodies with the FlexAble kit

Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share! 🙂

It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! 🙂 In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.

And you can ask for a free sample to test! :):):)

Open house at the LCI facility on 24th of September

Dear microscopy enthusiasts,

The Live Cell Imaging core facility is happy to invite you to a microscopy event on Tuesday, 24th of September.

You will have the opportunity to see Nikon ECLIPSE Ji Smart microscope in action!!!! A fast and easy to work with benchtop microscope with many ready to run experimental assays, AI powered automated imaging and instant image analysis!!!!

We are also showing the power of our CrEST V3DeepSIM super-resolution microscope, that offers double the resolution of a confocal microscope (down to aprox. 100 nm resolution in xy; 300 nm in z), even in thick samples. Flexible and can be used with any objectives, even low magnification air objectives!!!!

Do you want to image large, cleared samples? No problem, come to our demo and see our fast imaging light sheet microscope from M Squared in action!

Program

9:30-12.15 Open house: Come and ask questions about microscopes at our facility! Drop in.

9.30-10.00 Public demonstration of CrEST V3 spinning disk microscope

10.00-10.45 Public demonstration of the Nikon ECLIPSE Ji Smart Imaging microscope

10.45-11.00 Coffee

11.00-11.45 Public demonstration of the CrEST V3 DeepSIM super-resolution microscope

11.45- 12.15 Public demonstration of the Light sheet microscope from M Squared

13.00- 17.00 Private demonstration (bring your own sample) on ECLIPSE Ji and CrEST V3 DeepSIM

Registration is mandatory for private demonstration. Register by sending an email to gabriela.imreh@ki.se latest on 23^rd of Sept. Mention which instrument you would like to have the private demonstration on.

Venue: Flemingsberg Campus Neo, Karolinska Institute, Blickagången 16, Floor 7.

Please call us so we can let you in. (Gabriela Imreh 0707480761; Sylvie Le Guyader: 0737335008; Jianjang Hu: 0729087375)

Please share this event with friends and colleagues who may also be interested in attending this event.

Free online Microtutor course about fluorescence microscopy

Dear everyone

Microtutor Live will soon start its first flipped classroom version of its online Fluorescence microscopy course. I am happy to be one of the instructors for Europe! See below more info and how to apply! 🙂

Fluorescence Microscopy is a common technique in biological research, yet few researchers receive formal training on how to use it effectively. Microtutor, an interactive and free educational website, addresses this gap by offering self-guided courses on microscopy fundamentals. Students enroll themselves and work through the course at their own pace. Since the release of the first course on fluorescence microscopy in June, >600 students from all over the world have enrolled.

This fall, there is now going to be a special Microtutor LIVE version of the fluorescence microscopy course that includes instructor support instructor support. In this version, the students are assigned virtual course content each week to go through on their own, and then meet weekly with instructors over Zoom for Q&A. They can also attend virtual guest lectures each week, which will cover applications of the content. At the end, students can submit an optional individual project in the form of a microscopy experiment proposal to receive a certificate of completion for the course.

Students can learn more information and apply to join here.