Workshop – The Live Cell Imaging facility

Live demo of the Nikon Mizar TILT light sheet

On the 22nd of September at 10, you get a chance to see and try the latest light sheet on the market: the Nikon Mizar TILT.

- Easy to use stage top light sheet with only 1 objective
- Imaging of cell monolayer, cells in gels, organoids in gels, small organisms…
- Samples in glass bottom multiwell chamber slides
- Stage top incubator for live sample imaging
- Advantage of using the TILT light sheet
  - low phototoxicity and bleaching even with high resolution objective
  - excellent temporal and spatial resolution for live samples
- See here for more info.

This demo is only for a limited time (10 days) and in person only. Please let us know if you want to join the demo and if you want to try your own sample.

Live demo of the Crest spinning disk with DeepSIM

9^th of September, 10-12am: Live/zoom demo of the latest microscope acquired by the LCI facility: Crest spinning disk with DeepSIM (new Orion)

Ti2 microscope
Crest spinning disk (identical to the one on Tweety but with slightly different wavelengths)
DeepSIM: SIM is a fast super-resolution technique which is gentle for the sample (less bleaching, less photodamage for live samples) and offers double the resolution of a confocal microscope (down to 120nm resolution), even thick in samples. It is flexible and can be used with any objectives, even low magnification air objectives.
No specific sample preparation requirements
More information here

For the best experience, join the demo in person at the LCI facility (please apply by replying to this email) but if you cannot make it, you can also listen on Zoom.

The system has been purchased by the LCI so it is here to stay! 🙂

Please let us know if you want to join the demo in person and mention if you would like to try imaging your own sample with DeepSIM.

Super resolution STED event at Scilife in March

Nordic superresolution microscopy facility staff/researchers: STED

March 15th and 16th at 9-12 am. CONTACT: Hans Blom (hblom@kth.se)

Mar 15, 2022 9:00am

9:00-9:45am – BNMI info / STED intro [Hans Blom & Daniel Smeets (Leica)]
9:45-10:15am – STED sample prepping [Ulf Schwarz et al. (Leica)]
Break
10:30-11:00am – Input from a Nordic STED facility [Jonathan R. Brewer (DaMBIC)]
11:00-11:45am – STED demos [Luis Alvarez/Ulf Schwarz et al. (Leica)]
11:45-12:00am – Extra discussion time

https://kth-se.zoom.us/j/69376340781

Mar 16, 2022 9:00am

9:00-9:45am – Live-cell STED [Giovanna Coceano (KTH Stockholm)]
9:45-10:15am – Remote STED [Marko Lampe (ALMF/EMBL)]
Break
10:30-11:00am – STED-FCS [Erdinc Sezgin (KI Stockholm)]
11:00-11:45am – Advanced STED demos [Luis Alvarez/Ulf Schwarz et al. (Leica)]
11:45-12:00am – Extra discussion time

https://kth-se.zoom.us/j/67902191893

Listen to the lectures at the LCI facility microscopy course!

Starting from next week, the Live Cell Imaging core facility at KI will run its yearly microscopy course.

All the course lectures and some workshops will broadcasted live on Zoom, free of charge and there is no need to register.

Name: Microscopy: improve your imaging skills – from sample preparation to image analysis

Date: 24 Jan – 11 Feb 2022

Target audience:

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

The course covers the following topics:

Optics, image formation
Fluorescence, fluorophores
Bleedthrough
Anatomy of a microscope
Objectives and refraction index
Cameras and detectors
Noise and background, Bit depth and saturation
Multichannel imaging and spectral unmixing
Resolution and contrast
Sample preparation, Immunostaining
Nyquist sampling
Confocal and wide field settings
Speed, High throughput/content
Volume imaging, deconvolution
Clearing and expansion
Live cell imaging
Fourier
AI, Super Resolution microscopy
Colocalization
Data handling, OMERO.figure

On this page, you can find the course schedule (public activities are in blue) and the Zoom link to join. Scroll down to read the student testimonies! 😊

Hope you enjoy the Live Cell Imaging core facility microscopy course 2022! 😃

The LCI microscopy course 2022 is now open for registrations!

It is now time to apply to the intensive LCI microscopy course Jan/Feb 2022: Microscopy: improve your imaging skills – from sample preparation to image analysis

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that their knowledge is limited.

All the lectures at the LCI microscopy course will also broadcasted live online, free of charge and there is no need to register.

All details about the course including course schedule, how to apply, and how to follow the lectures are found here.

Scroll down to read the kind testimonies of our dear students! 😊

Hope you enjoy the LCI facility microscopy course 2022!

The first microscopy MOOC?

This MOOC for undergrad students by the University of Montpellier is about labeling and imaging vertebrate embryos.

All the videos are also available on their YouTube channel.

As far as I know, this is the first pure microscopy MOOC (Massive Open Online Course), dealing with sample preparation, imaging and data handling!

If you know of another microscopy MOOC (I know of some image analysis MOOC but not microscopy), please leave me a comment or sent me an email! 🙂

The LCI microscopy course 2021 starts next week! :)

As usual the lectures at the LCI microscopy course will broadcasted live online, free of charge and there is no need to register.

Title: Microscopy: improve your imaging skills – from sample preparation to image analysis

Applications are closed but all lectures will be broadcasted live and open to anyone without registration.

The course covers the following topics:

Optics, image formation, fluorescence, fluorophores, microscope and microscopy types
Objectives and refraction index, Cameras and detectors
Noise and background, Cameras and detectors, Bit depth and saturation, Multicolour imaging
Resolution and contrast, Sample preparation, Immunostaining
Nyquist sampling, Confocal and wide field settings, Scaling up and speeding up, High throughput/content
Volume imaging, deconvolution, multiphoton, Clearing and expansion
Live cell imaging, Fourier, AI, Super Resolution microscopy
Data handling, OMERO.figure, Requirements for image analysis, Colocalization
Image processing and analysis

Check the course schedule and details of how to join the Zoom webinars. Scroll down to read the kind testimonies of our dear students! 😊

Here is the course syllabus.

Hope you enjoy the LCI facility microscopy course 2021!

From Images to Knowledge virtual workshop

Nov 30th-Dec 2nd, Janelia Research Center organizes a great image analysis workshop with loads of goodies. Everything is online and free. There are in depth and more general workshops about Deep Learning with different freeware, programming and scripting of all sorts, Big data things… Check the program here.

CLIJ2: Open Computing Language and ImageJ2, GPU power for everyone

CLIJ2 allows you to use ImageJ/Fiji on GPU instead if CPU processing, so much faster! 🙂

Here is a nice article about what CLIJ2 can do. By the way, this article is published on a new imaging forum called FocalPlane. Check it out! And here is the presentation of how to use CLIJ2 at one of the recent Neubias event in May 2020.

If you have an analysis pipeline built in Fiji, Icy or Matlab and processing takes a long time, CLIJ2 will help you a lot.

Broadcasted lectures from the LCI microscopy course and private demos of light sheet and cameras

Our course starts tomorrow! 😀

Target audience:

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples and want to improve their skills.

Registrations are closed but all lectures are open to everyone without registration.

The schedule and details of the venue are here.
All lectures are also available online live. The link and instructions to watch are here.
Make sure you check the schedule in case of last minutes changes.

If you are in Sweden, you are welcome to try some of the equipment on demo with your own sample.

To book at timeslot, please contact the responsible person directly.

Light sheet microscope from M2Lasers: Valentina Loschiavo Valentina.Loschiavo(at)m2lasers.com
- Fast imaging of large sample
- Overview function to navigate in the sample and find the region of interest
- 800x800um field of view with 1um min resolution
- Any immersion media
- Sample size up to centimetres
Wide Field microscope from Nikon with 3 different Andor cameras: Oliver Garner (oliver.garner(at)bergmanlabora.se)
- Nikon Ti2 microscope with 4 times larger field of view
- A front illuminated sCMOS camera: Good sensitivity and resolution, great speed, but a greyish background (Andor Zyla 4.2)
- A back-illuminated EM-CCD camera: highly sensitive camera with very dark background, but lower resolution and lower speed (Andor 897U)
- A back-illuminated sCMOS camera: same sensitivity and low background as an EM-CCD but better resolution and speed (Andor Sona)