Dear all
An interesting whole-day 3D imaging symposium at Scilife on the 4th of December. Many really inspiring talks! 🙂
Tag: Learning
Time to apply to the LCI Microscopy course! :)
Dear all
You are now welcome to apply to join the LCI light microscopy course (21 Jan – 13 Feb 2026) ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).
The purpose of this fully online yet very applied course is to provide PhD students and researchers with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
-
- Prepare their sample and formulate their scientific question in a way that is suitable for reliable data extraction.
- Properly use the software and hardware available on the microscope they use in their lab/facility.
- Design their experiment based on image analysis requirements.
Application deadline 5th of November.
Course syllabus, description and registration. Please carefully check the eligibility criteria and make sure to include all required items in your application.
Those who cannot register to the course can anyway follow any of the lectures (in blue on the schedule) as they will be publicly broadcasted live on Zoom and accessible to anyone without registration. The schedule and zoom link are on the course page.
Kindly help us spread the word. 🙂
The LCI team
Online course for microscopy facility staff: “Magnify Your Impact: Train the Microscopy Trainer.”
Dr. Sylvie Le Guyader (sylvie.le.guyader@ki.se) and Ms. Rhonda Powell (rhondar@clemson.edu) invite you to apply to the online course: “Magnify Your Impact: Train the Microscopy Trainer.”
This practical, interactive, online course is intended for anyone with experience training people to use light microscopes, including Core Facility Staff and Industry Applications Specialists.
During the course, you will focus on improving your OWN training. By the end of the course, you will be able to:
- Write a list of learning objectives for your own microscopy training
- Incorporate new pedagogical tools in your microscopy training
- Evaluate the quality of your training using assessments and feedback
Please see the attached flyer for details and a link to the application, or visit https://tinyurl.com/magnifyyourimpactapplication
The application deadline is September 20, 2025. Applications will be reviewed in the order they are received. Questions about the application can be directed to Rhonda.
Flemingsberg science fun!
Do you want to know more about working at a Biotech companies? Meet the companies located on the Flemingsberg campus! Join the Flemingsberg Science breakfast meetings (in English) every wednesday in Novum.
Useful tips on the Nikon E-learning platform
Dear all
Have a quick look at Nikon’s e-learning platform! There are many useful tips and reminders of what we teach during the LCI training and course. 🙂
For example, here is a reminder of how refractive index mismatch distorts your images and when you should be concerned. 🙂
High Speed Multiphoton Imaging of Dynamic Processes In Vivo at Stockholm University
Intravital people might be interested in this event at the Intravital Microscopy Stockholm University facility:
The online LCI microscopy course: Microscopy: improve your imaging skills – from sample preparation to image analysis.
Dear all
It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28th of Jan) and runs until the 13th of February.
As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Here is a selection of what we will talk about:
- Optics and image formation,
- How microscopes work
- Objectives and refraction index
- Cameras and detectors, Noise and background, Bit depth and saturation
- Sample preparation, Immunostaining, Clearing
- Resolution and contrast, Nyquist sampling, how to choose microscope settings
- Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
- Image processing and analysis
Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.
Scroll down to read the kind testimonies of our dear students! 🙂
We hope that you will enjoy the LCI facility online microscopy course!
Kindly forward to anyone who might be interested.
The LCI team
Registrations to the LCI microscopy course 2025 will soon close!
There are a few spots left at our course (27 Jan – 14 Feb 2025): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits). Application deadline: 5th of November 2024.
This course is completely unique in that it is a highly hands-on course but because it runs completely remotely, your hands will be on your own microscope and your own sample. 😊
- To see how the course can help your microscopy project, check out the course schedule 2025 and the alumni testimonies on the course webpage.
- You can read the course syllabus and apply on this page. Make sure you check the eligibility criteria and click on the + sign at the top for more information.
- If you cannot apply to the course, you can anyway follow any of the lectures (in blue on the course schedule) as they will be publicly broadcasted live on Zoom and accessible to anyone without registration. The schedule and zoom link are available on the course page.
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
- assess and improve their sample so that it becomes suitable for data extraction from fluorescence images,
- make best use of the hardware available in their lab/facility,
- fully understand the acquisition parameters they need to set in their own microscope software,
- design their experiment from scientific question to image analysis using a strong knowledge base.
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
Gisele is hiring!
At the LCI, we have a long standing collaboration with Gisele Miranda, a great and kind image analyst affiliated to BIIF. Gisele is now starting her own image analysis lab! She is currently hiring 2 PhD students so if you know anyone interested, please spread the word. Here is what Gisele says:
I am excited to share that I have recently started my own research group as a SciLifeLab Fellow at KTH Royal Institute of Technology and the Science for Life Laboratory in Stockholm, Sweden. My group will focus on developing and applying cutting-edge Machine Learning techniques to deepen our understanding of cellular behavior and interactions. With a strong emphasis on generative AI, we aim to explore diverse data types, such as microscopy images and omics data, to simulate and predict biological behavior. Our work will focus on mapping complex relationships between single-cell and spatial features, driving new insights into the underlying architecture of cellular networks.
As part of building my group, I currently have two open PhD positions. I would deeply appreciate your help in spreading the word about these opportunities to potential candidates who are interested in working at the intersection of AI and biology.
https://www.kth.se/lediga-jobb/751352?l=en
https://www.kth.se/lediga-jobb/758900?l=en
Thank you for your support, and I look forward to staying connected as my group takes shape!
Best regards,
Gisele
Open house at the LCI facility on 24th of September
Dear microscopy enthusiasts,
The Live Cell Imaging core facility is happy to invite you to a microscopy event on Tuesday, 24th of September.
You will have the opportunity to see Nikon ECLIPSE Ji Smart microscope in action!!!! A fast and easy to work with benchtop microscope with many ready to run experimental assays, AI powered automated imaging and instant image analysis!!!!
We are also showing the power of our CrEST V3DeepSIM super-resolution microscope, that offers double the resolution of a confocal microscope (down to aprox. 100 nm resolution in xy; 300 nm in z), even in thick samples. Flexible and can be used with any objectives, even low magnification air objectives!!!!
Do you want to image large, cleared samples? No problem, come to our demo and see our fast imaging light sheet microscope from M Squared in action!
Program
9:30-12.15 Open house: Come and ask questions about microscopes at our facility! Drop in.
9.30-10.00 Public demonstration of CrEST V3 spinning disk microscope
10.00-10.45 Public demonstration of the Nikon ECLIPSE Ji Smart Imaging microscope
10.45-11.00 Coffee
11.00-11.45 Public demonstration of the CrEST V3 DeepSIM super-resolution microscope
11.45- 12.15 Public demonstration of the Light sheet microscope from M Squared
13.00- 17.00 Private demonstration (bring your own sample) on ECLIPSE Ji and CrEST V3 DeepSIM
Registration is mandatory for private demonstration. Register by sending an email to gabriela.imreh@ki.se latest on 23rd of Sept. Mention which instrument you would like to have the private demonstration on.
Venue: Flemingsberg Campus Neo, Karolinska Institute, Blickagången 16, Floor 7.
Please call us so we can let you in. (Gabriela Imreh 0707480761; Sylvie Le Guyader: 0737335008; Jianjang Hu: 0729087375)
Please share this event with friends and colleagues who may also be interested in attending this event.