Tag: Cool technique

Free, online microscopy course by the LCI core facility

Dear all

Kindly forward to anyone who might be interested.

It is my pleasure to invite you to follow our free (no registration) online microscopy course.

The course schedule and Zoom link are found our course webpage.

The public program (everything in blue in the course schedule) starts next week on Thursday 22th of Jan and runs until the 13th of February.

Here is a selection of what we will talk about:

  • Optics and image formation,
  • How microscopes work
  • Objectives and refraction index
  • Cameras and detectors, Noise and background, Bit depth and saturation
  • Sample preparation, Immunostaining, Clearing
  • Resolution and contrast, Nyquist sampling, how to choose microscope settings
  • Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
  • Image processing and analysis

All lectures will be broadcasted live on Zoom, recorded then posted the same day on our Youtube channel.

The course syllabus with the Learning Objectives can be downloaded here.

Do not miss the kind testimonies on our course webpage! 🙂

The LCI core facility team, Stockholm, Sweden

3D imaging symposium

Dear all

An interesting whole-day 3D imaging symposium at Scilife on the 4th of December. Many really inspiring talks! 🙂

Flemingsberg science fun!

Do you want to know more about working at a Biotech companies? Meet the companies located on the Flemingsberg campus! Join the Flemingsberg Science breakfast meetings (in English) every wednesday in Novum.

High Speed Multiphoton Imaging of Dynamic Processes In Vivo at Stockholm University

Intravital people might be interested in this event at the Intravital Microscopy Stockholm University facility:

Clearing live samples!

A couple of super cool papers about transiently clearing live samples to obtain better images!

On Bioarxiv

In Science

The online LCI microscopy course: Microscopy: improve your imaging skills – from sample preparation to image analysis.

Dear all

It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28th of Jan) and runs until the 13th of February.

As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

Here is a selection of what we will talk about:

  • Optics and image formation,
  • How microscopes work
  • Objectives and refraction index
  • Cameras and detectors, Noise and background, Bit depth and saturation
  • Sample preparation, Immunostaining, Clearing
  • Resolution and contrast, Nyquist sampling, how to choose microscope settings
  • Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
  • Image processing and analysis

Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.

Scroll down to read the kind testimonies of our dear students! 🙂

We hope that you will enjoy the LCI facility online microscopy course!

Kindly forward to anyone who might be interested.

The LCI team

Correlative microscopy webinar

For those of you who are interested in Correlative microscopy where one overlays the data acquired from different imaging modalities, Zeiss organises a webinar about combining light microscopy, EM microscopy and Z ray data for very large samples.

Here is the link.

 

Direct label of primary antibodies with the FlexAble kit

Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share! 🙂

It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! 🙂 In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.

And you can ask for a free sample to test! :):):)

 

Gisele is hiring!

At the LCI, we have a long standing collaboration with Gisele Miranda, a great and kind image analyst affiliated to BIIF. Gisele is now starting her own image analysis lab! She is currently hiring 2 PhD students so if you know anyone interested, please spread the word. Here is what Gisele says:

I am excited to share that I have recently started my own research group as a SciLifeLab Fellow at KTH Royal Institute of Technology and the Science for Life Laboratory in Stockholm, Sweden. My group will focus on developing and applying cutting-edge Machine Learning techniques to deepen our understanding of cellular behavior and interactions. With a strong emphasis on generative AI, we aim to explore diverse data types, such as microscopy images and omics data, to simulate and predict biological behavior. Our work will focus on mapping complex relationships between single-cell and spatial features, driving new insights into the underlying architecture of cellular networks.

As part of building my group, I currently have two open PhD positions. I would deeply appreciate your help in spreading the word about these opportunities to potential candidates who are interested in working at the intersection of AI and biology.

https://www.kth.se/lediga-jobb/751352?l=en

https://www.kth.se/lediga-jobb/758900?l=en

Thank you for your support, and I look forward to staying connected as my group takes shape!

Best regards,
Gisele

BIIF is hiring!

The BioImage Informatics facility at Scilife is hiring 2 image analysts! Here are the ads:

Open Positions @BIIF

Please contact BIIF directly if you have any question.

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