Dear all
An interesting whole-day 3D imaging symposium at Scilife on the 4th of December. Many really inspiring talks! 🙂
Tag: Cool technique
Flemingsberg science fun!
Do you want to know more about working at a Biotech companies? Meet the companies located on the Flemingsberg campus! Join the Flemingsberg Science breakfast meetings (in English) every wednesday in Novum.
High Speed Multiphoton Imaging of Dynamic Processes In Vivo at Stockholm University
Intravital people might be interested in this event at the Intravital Microscopy Stockholm University facility:
Clearing live samples!
A couple of super cool papers about transiently clearing live samples to obtain better images!
On Bioarxiv
- In Vivo Optical Clearing of Mammalian Brain, Franzesi et al
- Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo, Inagaki et al
In Science
The online LCI microscopy course: Microscopy: improve your imaging skills – from sample preparation to image analysis.
Dear all
It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28th of Jan) and runs until the 13th of February.
As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Here is a selection of what we will talk about:
- Optics and image formation,
- How microscopes work
- Objectives and refraction index
- Cameras and detectors, Noise and background, Bit depth and saturation
- Sample preparation, Immunostaining, Clearing
- Resolution and contrast, Nyquist sampling, how to choose microscope settings
- Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
- Image processing and analysis
Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.
Scroll down to read the kind testimonies of our dear students! 🙂
We hope that you will enjoy the LCI facility online microscopy course!
Kindly forward to anyone who might be interested.
The LCI team
Correlative microscopy webinar
For those of you who are interested in Correlative microscopy where one overlays the data acquired from different imaging modalities, Zeiss organises a webinar about combining light microscopy, EM microscopy and Z ray data for very large samples.
Here is the link.
Direct label of primary antibodies with the FlexAble kit
Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share! 🙂
It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! 🙂 In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.
And you can ask for a free sample to test! :):):)
Gisele is hiring!
At the LCI, we have a long standing collaboration with Gisele Miranda, a great and kind image analyst affiliated to BIIF. Gisele is now starting her own image analysis lab! She is currently hiring 2 PhD students so if you know anyone interested, please spread the word. Here is what Gisele says:
I am excited to share that I have recently started my own research group as a SciLifeLab Fellow at KTH Royal Institute of Technology and the Science for Life Laboratory in Stockholm, Sweden. My group will focus on developing and applying cutting-edge Machine Learning techniques to deepen our understanding of cellular behavior and interactions. With a strong emphasis on generative AI, we aim to explore diverse data types, such as microscopy images and omics data, to simulate and predict biological behavior. Our work will focus on mapping complex relationships between single-cell and spatial features, driving new insights into the underlying architecture of cellular networks.
As part of building my group, I currently have two open PhD positions. I would deeply appreciate your help in spreading the word about these opportunities to potential candidates who are interested in working at the intersection of AI and biology.
https://www.kth.se/lediga-jobb/751352?l=en
https://www.kth.se/lediga-jobb/758900?l=en
Thank you for your support, and I look forward to staying connected as my group takes shape!
Best regards,
Gisele
BIIF is hiring!
The BioImage Informatics facility at Scilife is hiring 2 image analysts! Here are the ads:
Open Positions @BIIF
- A permanent bioinformatician position (6-month trial period) located at Uppsala University, Department of Computer Science.
- Research Software Engineer position located at our KTH site in Stockholm Solna. This is a 1-year position, but there is a possibility to extend it to a permanent position after.
Please contact BIIF directly if you have any question.
Open house at the LCI facility on 24th of September
Dear microscopy enthusiasts,
The Live Cell Imaging core facility is happy to invite you to a microscopy event on Tuesday, 24th of September.
You will have the opportunity to see Nikon ECLIPSE Ji Smart microscope in action!!!! A fast and easy to work with benchtop microscope with many ready to run experimental assays, AI powered automated imaging and instant image analysis!!!!
We are also showing the power of our  CrEST V3DeepSIM super-resolution microscope, that offers double the resolution of a confocal microscope (down to  aprox. 100 nm resolution in xy; 300 nm in z), even in thick samples. Flexible and can be used with any objectives, even low magnification air objectives!!!!
Do you want to image large, cleared samples? No problem, come to our demo and see our fast imaging light sheet microscope from M Squared in action!
Program
9:30-12.15      Open house: Come and ask questions about microscopes at our facility! Drop in.
9.30-10.00         Public demonstration of CrEST V3 spinning disk microscope
10.00-10.45       Public demonstration of the Nikon ECLIPSE Ji Smart Imaging microscopeÂ
10.45-11.00 Â Â Â Â Â Â Coffee
11.00-11.45       Public demonstration of the CrEST V3 DeepSIM super-resolution microscope
11.45- 12.15 Â Â Â Â Public demonstration of the Light sheet microscope from M Squared
13.00- 17.00     Private demonstration (bring your own sample) on ECLIPSE Ji and CrEST V3 DeepSIM
Registration is mandatory for private demonstration. Register by sending an email to gabriela.imreh@ki.se latest on 23rd of Sept. Mention which instrument you would like to have the private demonstration on.
Venue: Flemingsberg Campus Neo, Karolinska Institute, Blickagången 16, Floor 7.
Please call us so we can let you in. (Gabriela Imreh 0707480761; Sylvie Le Guyader: 0737335008; Jianjang Hu: 0729087375)
Please share this event with friends and colleagues who may also be interested in attending this event.