LCI event – The Live Cell Imaging facility

Time to apply to the LCI Microscopy course! :)

Dear all

You are now welcome to apply to join the LCI light microscopy course (21 Jan – 13 Feb 2026) ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).

The purpose of this fully online yet very applied course is to provide PhD students and researchers with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:

1. Prepare their sample and formulate their scientific question in a way that is suitable for reliable data extraction.
2. Properly use the software and hardware available on the microscope they use in their lab/facility.
3. Design their experiment based on image analysis requirements.

Application deadline 5^th of November.

Course webpage.

Course syllabus, description and registration. Please carefully check the eligibility criteria and make sure to include all required items in your application.

Those who cannot register to the course can anyway follow any of the lectures (in blue on the schedule) as they will be publicly broadcasted live on Zoom and accessible to anyone without registration. The schedule and zoom link are on the course page.

Kindly help us spread the word. 🙂

The LCI team

Online course for microscopy facility staff: “Magnify Your Impact: Train the Microscopy Trainer.”

Dr. Sylvie Le Guyader (sylvie.le.guyader@ki.se) and Ms. Rhonda Powell (rhondar@clemson.edu) invite you to apply to the online course: “Magnify Your Impact: Train the Microscopy Trainer.”

This practical, interactive, online course is intended for anyone with experience training people to use light microscopes, including Core Facility Staff and Industry Applications Specialists.

During the course, you will focus on improving your OWN training. By the end of the course, you will be able to:

Write a list of learning objectives for your own microscopy training
Incorporate new pedagogical tools in your microscopy training
Evaluate the quality of your training using assessments and feedback

Please see the attached flyer for details and a link to the application, or visit https://tinyurl.com/magnifyyourimpactapplication

The application deadline is September 20, 2025. Applications will be reviewed in the order they are received. Questions about the application can be directed to Rhonda.

The online LCI microscopy course: Microscopy: improve your imaging skills – from sample preparation to image analysis.

Dear all

It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28^th of Jan) and runs until the 13^th of February.

As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

Here is a selection of what we will talk about:

Optics and image formation,
How microscopes work
Objectives and refraction index
Cameras and detectors, Noise and background, Bit depth and saturation
Sample preparation, Immunostaining, Clearing
Resolution and contrast, Nyquist sampling, how to choose microscope settings
Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
Image processing and analysis

Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.

Scroll down to read the kind testimonies of our dear students! 🙂

We hope that you will enjoy the LCI facility online microscopy course!

Kindly forward to anyone who might be interested.

The LCI team

Registrations to the LCI microscopy course 2025 will soon close!

There are a few spots left at our course (27 Jan – 14 Feb 2025): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits). Application deadline: 5th of November 2024.

This course is completely unique in that it is a highly hands-on course but because it runs completely remotely, your hands will be on your own microscope and your own sample. 😊

To see how the course can help your microscopy project, check out the course schedule 2025 and the alumni testimonies on the course webpage.
You can read the course syllabus and apply on this page. Make sure you check the eligibility criteria and click on the + sign at the top for more information.
If you cannot apply to the course, you can anyway follow any of the lectures (in blue on the course schedule) as they will be publicly broadcasted live on Zoom and accessible to anyone without registration. The schedule and zoom link are available on the course page.

The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:

assess and improve their sample so that it becomes suitable for data extraction from fluorescence images,
make best use of the hardware available in their lab/facility,
fully understand the acquisition parameters they need to set in their own microscope software,
design their experiment from scientific question to image analysis using a strong knowledge base.

The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.

The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.

Free online Microtutor course about fluorescence microscopy

Dear everyone

Microtutor Live will soon start its first flipped classroom version of its online Fluorescence microscopy course. I am happy to be one of the instructors for Europe! See below more info and how to apply! 🙂

Fluorescence Microscopy is a common technique in biological research, yet few researchers receive formal training on how to use it effectively. Microtutor, an interactive and free educational website, addresses this gap by offering self-guided courses on microscopy fundamentals. Students enroll themselves and work through the course at their own pace. Since the release of the first course on fluorescence microscopy in June, >600 students from all over the world have enrolled.

This fall, there is now going to be a special Microtutor LIVE version of the fluorescence microscopy course that includes instructor support instructor support. In this version, the students are assigned virtual course content each week to go through on their own, and then meet weekly with instructors over Zoom for Q&A. They can also attend virtual guest lectures each week, which will cover applications of the content. At the end, students can submit an optional individual project in the form of a microscopy experiment proposal to receive a certificate of completion for the course.

Students can learn more information and apply to join here.

The LCI open microscopy course starts on Monday! :)

Dear all

It is my pleasure to invite you to follow the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis.

The course starts next Monday (29 Jan) and runs until the 16^th of February.

As usual, all the course lectures are broadcasted live on Zoom. It is free of charge and you do not need to register.

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

Here is a selection of what we will talk about:

Optics and image formation,
Anatomy of a microscope
Objectives and refraction index
Cameras and detectors, Noise and background, Bit depth and saturation
Sample preparation, Immunostaining, Clearing and expansion
Resolution and contrast, Nyquist sampling, Microscope settings
Data handling, OMERO.figure, Requirements for image analysis, Colocalization
Image processing and analysis

Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Scroll down to read the kind testimonies of our dear students! 🙂

Here is the course syllabus.

For those who are on a far away time zome, we record all the live lectures and post them every evening on the LCI facility YouTube channel! 🙂

We hope that you will enjoy the LCI facility microscopy course!

Kindly forward to anyone who might be interested.

The LCI team

A few spots left at the LCI microscopy course 29/01-16/02 2024

There are a few spots left for the LCI core facility light microscopy course (29 Jan – 17 Feb 2024): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).

This course is completely unique in that it is a highly hands-on, but your hands will be on your own microscope and own sample. The course runs completely remotely! 😊

To see how the course can help your microscopy project, check the course webpage. Look at the course schedule 2024 and the alumni testimonies!
Also check the course syllabus to see the eligibility criteria. Also read what you will learn in the Course content and Intended Learning Outcomes sections.
If you cannot apply to the course, you can anyway follow any of the lectures (in blue on the schedule) as they will be publicly broadcasted live on Zoom and accessible to anyone without registration. The schedule and zoom link are available on the course page.

1) assess and improve their sample so that it becomes suitable for data extraction from fluorescence images,

2) make best use of the hardware available in their lab/facility,

3) fully understand the acquisition parameters they need to set in their own microscope software,

4) design their experiment from scientific question to image analysis using a strong knowledge base.

The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.

The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.

Time to register to the LCI microscopy course!

Dear all

It is time to enroll to the yearly light microscopy course run by the Live Cell imaging core facility (29 Jan – 17 Feb 2024): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).

Check the course schedule 2024 and the syllabus to see the course content and alumni testimonies.
Postdocs or registered PhD students can apply to the course.
Please read carefully the eligibility criteria in the syllabus and note that the last registration date is the 15^th of November.
If you cannot apply to the course, you can anyway follow any of the lectures (in blue on the schedule) as they will be publicly broadcasted live on Zoom and accessible to anyone without registration. The schedule and zoom link are on the course page.

The purpose of this course is to enable PhD students and researchers who have already and recently used a microscope to acquire images of fluorescent samples, to improve their microscopy skills.

The course is NOT aimed at training people to use the LCI facility microscopes. The focus is instead on providing the students with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:

1) prepare their sample and formulate their scientific question in a way that is suitable for data extraction from fluorescence images

2) properly use the hardware available in their lab/facility and 3) fully understand each parameter they need to set in the software in their lab/facility.

The aim is to provide the course students with the tools to acquire on ANY wide field, confocal or light sheet microscope, images of their samples that reliably answer their scientific question.

Please help us spread the word. 😃

Nordic Microscopy Symposium – 3rd-5th October

We are delighted to invite you to the Nordic microscopy symposium at the Live Cell Imaging core facility, Nikon Centre of Excellence at Karolinska Institutet, organised by BergmanLabora, Ramcon Denmark and Inter Instrument AS.

The symposium will highlight the great research done in the Nordics where microscopy is a key tool.
New exciting equipment from Nikon will be showcased including the AX/NSPARC super-resolution point confocal and the CrEST DeepSIM super-resolution spinning disk confocal.
We will also be launching the new Nikon Eclipse Ji benchtop assay instrument and demo the simpler CrEST Cicero spinning disk confocal.
The capability of these systems will be demonstrated during public sessions (3^rd of October) and in private sessions (on 4^th and 5^th October) where you are welcome to bring your own samples.

Venue: Neo, Flemingsberg Campus, Karolinska Institute: Erna Möller Lecture hall (Symposium), Live Cell Imaging (LCI) core facility (Demos)

3rd October

- 1000-1145 Public demo: Nikon AX R NSPARC super-resolution point-scanning confocal, CrEST X-Light V3 super-resolution spinning disk confocal, CrEST Cicero compact spinning disk
- 1000-1145 Open house at the Live Cell Imaging core facility (Drop in. Contact Sylvie Le Guyader)
- 1200-1300 Lunch (requires pre-registration)
- 1300-1305 Welcome
- 1305-1345 Zuzana Kadlecova (Cambridge Institute for Medical Research – UK): Lights! Camera! Uptake! Zooming in on Endocytosis with Quantitative TIRF-SIM Analysis.
- 1345-1410 Nicoline Dorothea Daugaard (Dept. Biochemistry and Molecular Biology, SDU – Denmark): Tracking T cells and their interactions with cancer cells in 3D using NIS-Elements General Analysis (GA3)
- 1410-1440 LCI short talks:
  - Chiara Annunziata (Karolinska Institute – Sweden): A pipeline for the multiparametric assessment of the anti ageing effects of candidate molecules
  - Andrea Coschiera (Karolinska Institute – Sweden): Primary cilia promote the differentiation of human neurons through the WNT signaling pathway
  - Natalie Geyer (Karolinska Institute – Sweden): Multiplex RNA in situ hybridisation for liver parenchyma characterization in a metastatic context
- 1440-1500 Coffee
- 1500-1525 Technical talk (Nikon): Microscopy Simplified, Nordic Launch of Nikon’s new ECLIPSE Ji
- 1525-1540 Staffan Strömblad (Karolinska Institute – Sweden): Multi-site assessment of reproducibility in high-content cell migration imaging data
- 1540-1605 Pieta Mattila (University of Turku – Finland): Advanced imaging methods to visualize lymphocyte activation

4th and 5th October

- 0830-1700 Private demos (bring your sample) Nikon AX R NSPARC super-resolution point-scanning confocal, CrEST X-Light V3 super-resolution spinning disk confocal, CrEST Cicero compact spinning disk
- 0830-1700 Open house at the Live Cell Imaging core facility (Drop in. Contact Sylvie Le Guyader)

Registration is mandatory and can be done via the following site: Nordic Microscopy Symposium – BergmanLabora.

Please share this event with friends and colleagues who may also be interested in attending.

Nordic microscopy symposium – Save the date!

Please join the Nordic microscopy symposium on the 3^rd of October at the Live Cell Imaging core facility, Nikon Centre of Excellence at Karolinska Institutet. The symposium is organised by BergmanLabora, Ramcon Denmark and Inter Instrument AS. Please save the date.

The event will highlight Nordic research where light microscopy is a key tool.

Several Nikon fast super resolution systems will also be available: Nikon AX confocal with NSPARC and Nikon/CrEST V3 spinning disk with DeepSIM.

Before and after the symposium, the capability of these systems will be demonstrated during public sessions (on the 3^rd of October) and in private sessions (booking required) where you are welcome to bring your own samples.

Preliminary schedule:

Tuesday 3rd October

1000-1045 AX R NSPARC + CrEST V3 with DeepSIM public demonstration (in parallel)

1100-1145 AX R NSPARC + CrEST V3 with DeepSIM public demonstration (in parallel)

1200-1300 Lunch (requires pre-registration)

1300-1305 Welcome

1305-1345 *Keynote: TBC

1345-1410 *Nicoline Dorothea Daugaard (Dept. Biochemistry and Molecular Biology, SDU – Denmark):

Tracking T cells and their interactions with cancer cells in 3D using NIS-Elements General Analysis (GA3)

1410-1440 *LCI short talks:

Chiara Annunziata (Karolinska Institutet – Sweden)

Andrea Coschiera (Karolinska Institutet – Sweden)