The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
Check out this webinar tomorrow by Rickardo Henriques, the inventor of SRRF (surf), post processing magic for all types of images!
*Title:* Open and accessible cutting-edge technology for super-resolution and machine-learning enabled microscopy
*When: *June 5th 4:00 pm CEST
*How to access/register*
And here’s a small teaser if you want to quickly see the topics we’ll cover.
Updates on the next events of the NEUBIAS Academy@Home Webinar series,
Newly confirmed events:
5 May: ilastik beyond pixel classification, by Anna Kreshuk and Dominik Kutra-
6 May: GPU-Accelerated Image Processing with CLIJ2, by Robert Haase
7 May: Interactive Bioimage Analysis with Python and Jupyter, by Guillaume Witz
Upcoming events open to registration:
LAST CHANCE TO REGISTER:
28 April: Introduction to nuclei segmentation with StarDist, by Martin Weigert et al
29 April: Quantitative Pathology and Bioimage Analysis: QuPath v0.2.0, By Pete Bankhead
30 April: Advanced Image Processing with MorphoLibJ, by David Legland
Two weeks after the opening of the Academy and of the registrations, Webinars and online courses have already attracted over 5,000 registrations!
The events are recorded and some are already available on the Youtube NEUBIAS Channel.
Furthermore, a thread will be opened in the image.sc Forum to report Q&As and to welcome further questions/comments for each event.
You’ll find more information here.
Neubias is back with new ideas! Neubias is the Network of European Bioimage Analysts and what they burn for is to help scientists analyze their images.
Possibly inspired by the Corona time, they will start an online school for image analysis based on video tutorials and online events.
Have a look at their new page called Neubias academy where they announce several events coming up in the next few months.
Our course starts tomorrow! 😀
Target audience:
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples and want to improve their skills.
Registrations are closed but all lectures are open to everyone without registration.
If you are in Sweden, you are welcome to try some of the equipment on demo with your own sample.
To book at timeslot, please contact the responsible person directly.
Ever wondered if that antibody you used throughout your whole PhD was actually also binding to something else than its supposed target protein?
Antibody validation in tissue staining is a very difficult task!
Here is a great step-by-step validation protocol published by EuroMabNet, a network of scientists who try to improve antibody validation.
And this paper gives a useful flow chart for antibody validation.
And here is the 5 pillars of antibody validation paper which explains what can be done to validate antibodies.
Imagine starting a study about some cool protein.
You find some useful articles on Google Scholar. In one paper, an antibody is mentioned. The name of the company that sold it to the authors is mentioned in the Material and Method but unfortunately that company has closed down or has been swallowed by one of the Pharma giants so you cannot order. Then you realize that the company was not producing any antibodies anyway, they were buying it from another company so there is no way to trace and buy the same antibody. Nightmare…
Then imagine that instead, the paper mentions the RRid number for that antibody. You do not know about what that is but you check and find this paper that explains it all.
Now suddenly, not only you can find on the Scicrunch website which company produces this antibody and which resells it so you can buy it, but you can also search pubmed for the RRid and find all the articles that mention it, opening your eyes to lots of results about your protein that have been published specifically with using that antibody. Now you can also check if the antibody gives consistent results!
And imagine being to do this for your favorite mouse model as well. See all publications that have mentioned your mouse RRId!
But it relies on you writing the RRid of your antibody or mouse in your next publication so think about it! 😀
Jennifer Watson’s Microcourses channel on You Tube is really recommended to anyone who uses a microscope.
Short targetted videos that will boil down the principles of light microscopy for biologist and help you understand what you are doing.
There are a few series, each of them with a few videos and the collection is constantly growing. Remember to subscribe so you get to know when they post a new one.
NEUBIAS, the Network of European BioImage Analysts, is delighted to announce two new Training Schools on BioImage Analysis:
TS14 for Early Career Investigators (Life Scientists: PhD candidates, Postdocs, Staff, …):
This training school will cover the basics of image analysis using ImageJ/Fiji, as well as image analysis workflow automation using ImageJ macro programming. In addition, it will be taught how to use the software package ilastik for machine learning based image segmentation and object classification, and how to integrate ilastik into ImageJ macro based workflows. Moreover, an overview of further relevant bioimage analysis software packages will be given and there will be ample time for “Work on Your Own Data” sessions assisted by experienced Analysts.
TS15 for BioImage Analysts (advanced level):
This school targets bioimage analysts, who are willing to enhance their professional scope and techniques for improving the quality of their analysis, at the same time as willing to contribute with their knowledge and experience to the school. Prerequisite is a proficiency in at least one programming language (we do not train coding). The school focuses on workflow designing. This year, we will have a particular emphasis on statistics for bioimage analysis and related tools e.g. R and Python libraries. In addition, we will overview machine & deep learning components.
The schools will be held in Bordeaux, Feb 29 – Mar 03 2019, hosted at the Centre Broca Nouvelle-Aquitaine by the Bordeaux Imaging Center (BIC) and the Interdisciplinary Institute for Neuroscience. The selected students will be able to attend the whole NEUBIAS conference as part of the training.
NEUBIAS schools are an excellent opportunity to learn from many experts in Bioimage Analysis (we are expecting >20 specialists at the event) and “…a great mix of intensive learning and community networking” (former trainee testimonial). All schools include practical sessions “Work on Your Own Data”, plenary seminars and a session on ethics in image analysis.
Applications are now open (TS14 = 25 seats, TS15 = 35 seats and ~10+ trainers per school). Within the COST framework (funders of NEUBIAS), we will offer up to 7 travel grants per school to applicants who qualify.
Application deadline: December 12th 2019 Selection notification: December 20th 2019
More information about schools (programme & trainers) and venue, travel & lodge available at our website:
eubias.org/NEUBIAS/training-schools/
eubias.org/NEUBIAS/training-schools/eci/ts14-bordeaux-2020/
eubias.org/NEUBIAS/training-schools/analysts/ts15-bordeaux-2020/
We kindly ask that you help us reach out to all potential interested applicants.
on behalf of all NEUBIAS members, local organizers (Florian Levet and Fabrice Cordelières), scientific organizers (Romain Guiet, Elnaz Fazeli, Christian Tischer, Kota Miura, Marion Louveaux), and NEUBIAS Training Leaders Gabriel Martins and Fabrice Cordelières.
The LCI will host a seminar about GlyCLICK, a new way to directly label primary antibodies and stop using secondaries: 6th of November at 13:00 in the Lipid seminar room in Neo, KI Flemingsberg. The seminar will be broadcasted live. Here is how to find us and here how to follow the seminar online.
Everyone agrees that it would be great to be able to label our samples without using secondary antibodies.
There are many kits to label primary antibodies directly with fluorophores. The main disadvantage compared to primary/secondary stainings is that direct labels are often weaker because the final primary/fluorophore ratio is too low. Using an amplification method like TSA (Tyramide Signal Amplification) leads to a loss of resolution.
Over the years users at the LCI have tried this kit, this kit and this one. They gave mixed results depending on the antibody but we keep looking! Come to the LCI seminar about a new direct labelling technique that uses Click chemistry.
It is now time to register to the LCI intensive microscopy course (Jan/Feb 2020). Check out the course schedule.
Loads of fun workshops, informative lectures, intense discussions and our popular Student Imaging Challenge workshop where students get direct feedback on how to improve their own sample preparation/experimental design.
We always run two courses in parallel:
As usual, all lectures are public and broadcasted live so you are welcome to just show up (how to find us) or watch remotely (how to connect) without registration. Check the program as it may be updated in case of (unlikely) last minute changes.
We welcome your feedback about the quality of the webinar and the content of the lectures.
