The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
Kromnigon makes fluorescent labels called FlexiStain that stick to biotin-labelled primary antibodies.
This means that you skip the species matching headache when using multiple antibodies and your labeling is faster! I have not tried myself but they offer free trial kits so give it a try! You are welcome to leave comments on this posts to tell us if it works or not! 🙂
Note: We have not had very good experience with these dyes. Check this post instead.
If you are tired of DAPI bleeding through your weak green channel but are stuck to using blue for the nucleus, you might want to give a try to Syto41.
Must better excitation efficiency at 405 nm and much narrower emission spectrum!
And Syto also comes in other colors. 🙂
Those who work with FACS might be aware of the very bright dyes called Brilliant Violet. They can also be used in microscopy. 🙂
BV dyes are all excited around 400 nm (same as blue dyes) but they can emit at much longer wavelengths, like red or far red. This means that if they are used together, they are excited at the same time and the colors are only separated based on the emission! But these dyes are very bright which is a great advantage.
Et voilà ! 🙂
How to easily label Golgi, cytoskeleton, endosomes, lysosomes… in live cells with CFP, GFP or RFP then be able to fix everything? It does sound like pure magic! Have a look here.
