The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share! 🙂
It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! 🙂 In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.
And you can ask for a free sample to test! :):):)
There are a few spots left at our course (27 Jan – 14 Feb 2025): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits). Application deadline: 5th of November 2024.
This course is completely unique in that it is a highly hands-on course but because it runs completely remotely, your hands will be on your own microscope and your own sample. 😊
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
Dear everyone
Microtutor Live will soon start its first flipped classroom version of its online Fluorescence microscopy course. I am happy to be one of the instructors for Europe! See below more info and how to apply! 🙂
Fluorescence Microscopy is a common technique in biological research, yet few researchers receive formal training on how to use it effectively. Microtutor, an interactive and free educational website, addresses this gap by offering self-guided courses on microscopy fundamentals. Students enroll themselves and work through the course at their own pace. Since the release of the first course on fluorescence microscopy in June, >600 students from all over the world have enrolled.
This fall, there is now going to be a special Microtutor LIVE version of the fluorescence microscopy course that includes instructor support instructor support. In this version, the students are assigned virtual course content each week to go through on their own, and then meet weekly with instructors over Zoom for Q&A. They can also attend virtual guest lectures each week, which will cover applications of the content. At the end, students can submit an optional individual project in the form of a microscopy experiment proposal to receive a certificate of completion for the course.
Students can learn more information and apply to join here.
Dear all
It is my pleasure to invite you to follow the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis.
The course starts next Monday (29 Jan) and runs until the 16th of February.
As usual, all the course lectures are broadcasted live on Zoom. It is free of charge and you do not need to register.
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Here is a selection of what we will talk about:
Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Scroll down to read the kind testimonies of our dear students! 🙂
Here is the course syllabus.
For those who are on a far away time zome, we record all the live lectures and post them every evening on the LCI facility YouTube channel! 🙂
We hope that you will enjoy the LCI facility microscopy course!
Kindly forward to anyone who might be interested.
The LCI team
There are a few spots left for the LCI core facility light microscopy course (29 Jan – 17 Feb 2024): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).
This course is completely unique in that it is a highly hands-on, but your hands will be on your own microscope and own sample. The course runs completely remotely! 😊
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
1) assess and improve their sample so that it becomes suitable for data extraction from fluorescence images,
2) make best use of the hardware available in their lab/facility,
3) fully understand the acquisition parameters they need to set in their own microscope software,
4) design their experiment from scientific question to image analysis using a strong knowledge base.
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
mCherry is a very popular red fluorescent protein. However it has several disadvantages:
mCherry has now been evolved into mCherry-XL with several improvements:
Here is the paper. Therefore you should consider mCherry-XL for your future tagging with red fluorescence proteins.
It is now time to apply to the intensive LCI microscopy course Jan/Feb 2022: Microscopy: improve your imaging skills – from sample preparation to image analysis
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that their knowledge is limited.
All the lectures at the LCI microscopy course will also broadcasted live online, free of charge and there is no need to register.
All details about the course including course schedule, how to apply, and how to follow the lectures are found here.
Scroll down to read the kind testimonies of our dear students! 😊
Hope you enjoy the LCI facility microscopy course 2022!
Fluorophores are in constant development.
There is now a new generation of Alexa Fluor fluorophores: the Alexa Fluor Plus. They can be purchased already coupled to secondary antibodies on the ThermoFisher website.
Brilliant violet antibodies are also very bright and despite their name, they come in all colours. The can be purchased from Biolegend which produces the fluorophore.
Another excellent source of bright fluorophores is the Janelia Fluor dyes. They can be purchased coupled to antibodies from the NovusBio website.
As usual the lectures at the LCI microscopy course will broadcasted live online, free of charge and there is no need to register.
Title: Microscopy: improve your imaging skills – from sample preparation to image analysis
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Applications are closed but all lectures will be broadcasted live and open to anyone without registration.
The course covers the following topics:
Check the course schedule and details of how to join the Zoom webinars. Scroll down to read the kind testimonies of our dear students! 😊
Here is the course syllabus.
Hope you enjoy the LCI facility microscopy course 2021!
Fluorophores are evolving fast! Here is a paper about a bunch of new fluorophores isolated from the jelly fish Aequoria. This includes the brightest fluorophore ever isolated: a new green fluorescent protein called AausFP1 that is almost 5 times brigther than GFP! Respect! 🙂
