Image – Page 14 – The Live Cell Imaging facility

Six images acquired at the LCI at the Sven-Harrys museum!

The golden Sven-Harrys museum at the heart of Stockholm is currently exhibiting beautiful microscopy images. We proudly counted 6 of them that were acquired with the LCI microscopes! Go and have a look! 🙂

Alyona’s videos

Alyona Minina from the Confocal microscopy facility at SLU came up with really nice videos about Zen and how to use Zeiss confocals

Have a look!

Magic clarity :)

Remember that with passive clarity, you can make most tissues transparent in order to image them.

The challenge may be to get the antibody to penetrate fully if your tissue is very thick but clarity removes lipid so it also helps with antibody penetration. If your protein expresses a fluorescent protein, you skip this problem and clarity preserves fluorescence.

You can then mount your tissue and image it on our microscopes! 🙂 It’s magic!! 🙂

Confocal endoscopy talk in Uppsala

Interesting seminars in Uppsala. Apparently this endoscope can go inside our bodies and has has tiny objective of only 300um!

They will also present at KI (if you are interested contact Gilles Cestelli gilles(at)maunakeatech(dot)com)

Imaging job in Finland :)

Postdoc and facility manager at once in Helsinki!

Check it out here!

Microscopy workshop in Stockholm

Our infamous Hans Blom from the Advanced Light Microscopy facility at Scilife, is organizing a workshop at the end of this month.

Light sheet, super resolution and FCS on the menu, talks and demos.

Have a look at the program. 🙂

Good news on the glass bottom plates and chamber front!

There are loads of multiwell plates and chamber slides on the market but it is hard to get any that fulfill these criteria:

They do not kill the cells (unfortunately, this is not a joke!)
They do not leak (no joke either! Sigh…)
They have a low skirt: so they can be completely imaged (including the outer area of outer wells) with our Nikon 20x air, NA 0.75, WD 1mm objectives. The ‘skirt’ is the rim at the edge of the plate. A high skirt raises the plate so that it becomes impossible to image the wells at the edges of the plate as the objective bumps into the insert edge.
They have a #1.5 (=170um thick) glass coverslip bottom which is thickness any objective from any manufacturer is specified for.
They don’t cost a bomb.

Zell Kontakt is a German company that makes fantastic glass bottom dishes and plate (absolutely no commercial interests!) fulfilling ALL these criteria and are very flat! If you ask kindly, you may even get a free sample to test! 🙂

If you are at KI and need to write an exemption, just come and ask us for our template. 🙂

Job opening at Nikon Europe :)

Do you want to apply your microscopy knowledge in a commercial setting and join a company with a dynamic vision for the future?

Nikon Instruments is a world-renowned company that provides cutting edge technologies for optical instrumentation. We offer a creative working environment with highly motivated colleagues, team spirit, and comprehensive benefits and ongoing professional development opportunities.

Within our Nikon Instruments Europe Headquarters, a vacancy has arisen in our European Product Support Group (PSG) for an experienced European Product Specialist to join the team. The PSG team plays a key role in proactively coordinating the microscopy information flow across Europe concerning technical know-how and applications, including customer insight & commercial win-loss experiences.

Specifically, in this role, the successful candidate will be an ambassador for the Pan-European PSG team’s initiatives and strategic objectives, through providing a technical and commercially focused support programme that facilitates multiple communication channels, and adequately supports the local business units in each country to achieve net sales targets.

JOB TITLE: European product specialist

OVERALL RESPONSIBILITY:

As product specialist for the European headquarters, the overall responsibilities are to develop, implement, and plan for products mainly in the super resolution and laser application areas. These responsibilities include planning of product introductions, information management of products/systems, and helping achieve sales goals supporting our local teams, as well as technical and training roles with respect to these systems products.

Planning responsibilities include forecasting and management of vendor products based on market requirements and market trends. These also include competitive analyses, feature/benefit analyses, and interaction with partner vendors in the design, marketing, pricing, and applications of vendor products integrated into Nikon systems products.

Product development responsibilities include communication and training with the factory design and planning teams to implement new systems products, and provide feedback to the factory for competitive analysis and forecasting, in order to strengthen the overall systems product lines.

Information management responsibilities include associated training and marketing of systems products to Nikon team members and customers, and to act as a technical liaison when assisting the sales department in key sales demonstrations, installations, and technical trainings.

We are looking for a candidate with experience in laser based microscopy, preferably in super resolution systems such as STORM and SIM, and with experience in dealing with microscopy brands either from a research standpoint or as commercial or specialist experience.

If you are interested, please contact laura.schneider@nikon.com

Brilliant violet dyes

Those who work with FACS might be aware of the very bright dyes called Brilliant Violet. They can also be used in microscopy. 🙂

BV dyes are all excited around 400 nm (same as blue dyes) but they can emit at much longer wavelengths, like red or far red. This means that if they are used together, they are excited at the same time and the colors are only separated based on the emission! But these dyes are very bright which is a great advantage.

Et voilà! 🙂