The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share! 🙂
It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! 🙂 In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.
And you can ask for a free sample to test! :):):)
There are a few spots left at our course (27 Jan – 14 Feb 2025): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits). Application deadline: 5th of November 2024.
This course is completely unique in that it is a highly hands-on course but because it runs completely remotely, your hands will be on your own microscope and your own sample. 😊
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
Dear all
It is my pleasure to invite you to follow the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis.
The course starts next Monday (29 Jan) and runs until the 16th of February.
As usual, all the course lectures are broadcasted live on Zoom. It is free of charge and you do not need to register.
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Here is a selection of what we will talk about:
Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Scroll down to read the kind testimonies of our dear students! 🙂
Here is the course syllabus.
For those who are on a far away time zome, we record all the live lectures and post them every evening on the LCI facility YouTube channel! 🙂
We hope that you will enjoy the LCI facility microscopy course!
Kindly forward to anyone who might be interested.
The LCI team
There are a few spots left for the LCI core facility light microscopy course (29 Jan – 17 Feb 2024): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).
This course is completely unique in that it is a highly hands-on, but your hands will be on your own microscope and own sample. The course runs completely remotely! 😊
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
1) assess and improve their sample so that it becomes suitable for data extraction from fluorescence images,
2) make best use of the hardware available in their lab/facility,
3) fully understand the acquisition parameters they need to set in their own microscope software,
4) design their experiment from scientific question to image analysis using a strong knowledge base.
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
Spatial transcriptomics techniques are booming! It is now possible to identify where RNAs are located within a tissue! At the LCI facility, we routinely image samples labelled with RNAscope. If you use RNAscope, you might be interested in this workshop where you will learn how to label thick samples with RNAscope, and this symposium about automated RNAscope.
Additionally, we can now offer the 10x Genomics Visium technology on the South Campus, as a collaboration between 4 core facilities in Flemingsberg: LCI, FENO, SICOF and BEA core facilities!
Here is the workflow:
Come and talk to us about your Spatial Transcriptomics project!
Dear all
It is time to enroll to the yearly light microscopy course run by the Live Cell imaging core facility (29 Jan – 17 Feb 2024): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).
The purpose of this course is to enable PhD students and researchers who have already and recently used a microscope to acquire images of fluorescent samples, to improve their microscopy skills.
The course is NOT aimed at training people to use the LCI facility microscopes. The focus is instead on providing the students with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
1) prepare their sample and formulate their scientific question in a way that is suitable for data extraction from fluorescence images
2) properly use the hardware available in their lab/facility and 3) fully understand each parameter they need to set in the software in their lab/facility.
The aim is to provide the course students with the tools to acquire on ANY wide field, confocal or light sheet microscope, images of their samples that reliably answer their scientific question.
Please help us spread the word. 😃
Two really nice symposia/workshop on clearing and expansion microscopy next week!
mCherry is a very popular red fluorescent protein. However it has several disadvantages:
mCherry has now been evolved into mCherry-XL with several improvements:
Here is the paper. Therefore you should consider mCherry-XL for your future tagging with red fluorescence proteins.
Nordic superresolution microscopy facility staff/researchers: STED
March 15th and 16th at 9-12 am. CONTACT: Hans Blom (hblom@kth.se)
Mar 15, 2022 9:00am
https://kth-se.zoom.us/j/69376340781
Mar 16, 2022 9:00am
Starting from next week, the Live Cell Imaging core facility at KI will run its yearly microscopy course.
All the course lectures and some workshops will broadcasted live on Zoom, free of charge and there is no need to register.
Name: Microscopy: improve your imaging skills – from sample preparation to image analysis
Date: 24 Jan – 11 Feb 2022
Target audience:
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
The course covers the following topics:
On this page, you can find the course schedule (public activities are in blue) and the Zoom link to join. Scroll down to read the student testimonies! 😊
Hope you enjoy the Live Cell Imaging core facility microscopy course 2022! 😃
