The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share! 🙂
It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! 🙂 In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.
And you can ask for a free sample to test! :):):)
There are a few spots left at our course (27 Jan – 14 Feb 2025): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits). Application deadline: 5th of November 2024.
This course is completely unique in that it is a highly hands-on course but because it runs completely remotely, your hands will be on your own microscope and your own sample. 😊
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
Dear everyone
Microtutor Live will soon start its first flipped classroom version of its online Fluorescence microscopy course. I am happy to be one of the instructors for Europe! See below more info and how to apply! 🙂
Fluorescence Microscopy is a common technique in biological research, yet few researchers receive formal training on how to use it effectively. Microtutor, an interactive and free educational website, addresses this gap by offering self-guided courses on microscopy fundamentals. Students enroll themselves and work through the course at their own pace. Since the release of the first course on fluorescence microscopy in June, >600 students from all over the world have enrolled.
This fall, there is now going to be a special Microtutor LIVE version of the fluorescence microscopy course that includes instructor support instructor support. In this version, the students are assigned virtual course content each week to go through on their own, and then meet weekly with instructors over Zoom for Q&A. They can also attend virtual guest lectures each week, which will cover applications of the content. At the end, students can submit an optional individual project in the form of a microscopy experiment proposal to receive a certificate of completion for the course.
Students can learn more information and apply to join here.
Good news! The LCI facility is now part of the Swedish Eurobioimaging node! 🙂
This means that you can apply for funds to come and get trained, do some job shadowing or image at the LCI core facility!
The Swedish Eurobioimaging node is even partner in special European funding programs for Agroecology, and Cancer research.
We are looking forward to welcoming you to our facility! 🙂
There are a few spots left for the LCI core facility light microscopy course (29 Jan – 17 Feb 2024): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits).
This course is completely unique in that it is a highly hands-on, but your hands will be on your own microscope and own sample. The course runs completely remotely! 😊
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
1) assess and improve their sample so that it becomes suitable for data extraction from fluorescence images,
2) make best use of the hardware available in their lab/facility,
3) fully understand the acquisition parameters they need to set in their own microscope software,
4) design their experiment from scientific question to image analysis using a strong knowledge base.
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
We are delighted to invite you to the Nordic microscopy symposium at the Live Cell Imaging core facility, Nikon Centre of Excellence at Karolinska Institutet, organised by BergmanLabora, Ramcon Denmark and Inter Instrument AS.
Venue: Neo, Flemingsberg Campus, Karolinska Institute: Erna Möller Lecture hall (Symposium), Live Cell Imaging (LCI) core facility (Demos)
3rd October
4th and 5th October
Registration is mandatory and can be done via the following site: Nordic Microscopy Symposium – BergmanLabora.
Please share this event with friends and colleagues who may also be interested in attending.
Two really nice symposia/workshop on clearing and expansion microscopy next week!
I am wowed at the images published recently in BioArxiv in the Zero-shot deconvolution networks paper. It will be interesting to see the peer-reviewed paper.
I would be interested in visualizing the amount of errors made by the network. A simple way to do it is to acquire a short time lapse (eg 10 images) of a fixed sample, run it through the network and see which structures are stably identified and which change from frame to frame. 🙂
Light-Seq is a new pretty cool technique for highly multiplexed sequencing of RNA in tissue sections using light. This technique is highly sensitive, highly spatially resolved and because it does not destroy the tissue, it can be combined with protein labelling (genetic or by immunolabelling).
On one of our single-point confocal/spinning disk/widefield system at the LCI facility, we have a device called Primo (DMD + UV laser) which can be used to run this technique! 🙂
Let us know if you would like to set up LightSeq at the LCI core facility!
On the 22nd of September at 10, you get a chance to see and try the latest light sheet on the market: the Nikon Mizar TILT.
This demo is only for a limited time (10 days) and in person only. Please let us know if you want to join the demo and if you want to try your own sample.
