The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
Dear all
Have a quick look at Nikon’s e-learning platform! There are many useful tips and reminders of what we teach during the LCI training and course. 🙂
For example, here is a reminder of how refractive index mismatch distorts your images and when you should be concerned. 🙂
Intravital people might be interested in this event at the Intravital Microscopy Stockholm University facility:
Dear all
It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28th of Jan) and runs until the 13th of February.
As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Here is a selection of what we will talk about:
Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.
Scroll down to read the kind testimonies of our dear students! 🙂
We hope that you will enjoy the LCI facility online microscopy course!
Kindly forward to anyone who might be interested.
The LCI team
Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share! 🙂
It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! 🙂 In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.
And you can ask for a free sample to test! :):):)
Dear microscopy enthusiasts,
The Live Cell Imaging core facility is happy to invite you to a microscopy event on Tuesday, 24th of September.
You will have the opportunity to see Nikon ECLIPSE Ji Smart microscope in action!!!! A fast and easy to work with benchtop microscope with many ready to run experimental assays, AI powered automated imaging and instant image analysis!!!!
We are also showing the power of our  CrEST V3DeepSIM super-resolution microscope, that offers double the resolution of a confocal microscope (down to  aprox. 100 nm resolution in xy; 300 nm in z), even in thick samples. Flexible and can be used with any objectives, even low magnification air objectives!!!!
Do you want to image large, cleared samples? No problem, come to our demo and see our fast imaging light sheet microscope from M Squared in action!
Program
9:30-12.15      Open house: Come and ask questions about microscopes at our facility! Drop in.
9.30-10.00         Public demonstration of CrEST V3 spinning disk microscope
10.00-10.45       Public demonstration of the Nikon ECLIPSE Ji Smart Imaging microscopeÂ
10.45-11.00 Â Â Â Â Â Â Coffee
11.00-11.45       Public demonstration of the CrEST V3 DeepSIM super-resolution microscope
11.45- 12.15 Â Â Â Â Public demonstration of the Light sheet microscope from M Squared
13.00- 17.00     Private demonstration (bring your own sample) on ECLIPSE Ji and CrEST V3 DeepSIM
Registration is mandatory for private demonstration. Register by sending an email to gabriela.imreh@ki.se latest on 23rd of Sept. Mention which instrument you would like to have the private demonstration on.
Venue: Flemingsberg Campus Neo, Karolinska Institute, Blickagången 16, Floor 7.
Please call us so we can let you in. (Gabriela Imreh 0707480761; Sylvie Le Guyader: 0737335008; Jianjang Hu: 0729087375)
Please share this event with friends and colleagues who may also be interested in attending this event.
Dear everyone
Microtutor Live will soon start its first flipped classroom version of its online Fluorescence microscopy course. I am happy to be one of the instructors for Europe! See below more info and how to apply! 🙂
Fluorescence Microscopy is a common technique in biological research, yet few researchers receive formal training on how to use it effectively. Microtutor, an interactive and free educational website, addresses this gap by offering self-guided courses on microscopy fundamentals. Students enroll themselves and work through the course at their own pace. Since the release of the first course on fluorescence microscopy in June, >600 students from all over the world have enrolled.
This fall, there is now going to be a special Microtutor LIVE version of the fluorescence microscopy course that includes instructor support instructor support. In this version, the students are assigned virtual course content each week to go through on their own, and then meet weekly with instructors over Zoom for Q&A. They can also attend virtual guest lectures each week, which will cover applications of the content. At the end, students can submit an optional individual project in the form of a microscopy experiment proposal to receive a certificate of completion for the course.
Students can learn more information and apply to join here.Â
Good news! The LCI facility is now part of the Swedish Eurobioimaging node! 🙂
This means that you can apply for funds to come and get trained, do some job shadowing or image at the LCI core facility!
The Swedish Eurobioimaging node is even partner in special European funding programs for Agroecology, and Cancer research.
We are looking forward to welcoming you to our facility! 🙂
Participate to a online evening of fun activities with other students who use microscopy across the world! 🙂
Scan the code to register and choose Other for microscopy society.
Dear all
It is my pleasure to invite you to follow the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis.
The course starts next Monday (29 Jan) and runs until the 16th of February.
As usual, all the course lectures are broadcasted live on Zoom. It is free of charge and you do not need to register.
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Here is a selection of what we will talk about:
Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Scroll down to read the kind testimonies of our dear students! 🙂
Here is the course syllabus.
For those who are on a far away time zome, we record all the live lectures and post them every evening on the LCI facility YouTube channel! 🙂
We hope that you will enjoy the LCI facility microscopy course!
Kindly forward to anyone who might be interested.
The LCI team
Spatial transcriptomics techniques are booming! It is now possible to identify where RNAs are located within a tissue! At the LCI facility, we routinely image samples labelled with RNAscope. If you use RNAscope, you might be interested in this workshop where you will learn how to label thick samples with RNAscope, and this symposium about automated RNAscope.
Additionally, we can now offer the 10x Genomics Visium technology on the South Campus, as a collaboration between 4 core facilities in Flemingsberg: LCI, FENO, SICOF and BEA core facilities!
Here is the workflow:
Come and talk to us about your Spatial Transcriptomics project!
