2021 – The Live Cell Imaging facility

The Live Cell Imaging facility is Nikon Center of Excellence – Symposium and open house – tomorrow 10/11

Tomorrow, we will celebrate us being Nikon Center of Excellence with great microscopy talks, a live demo of the latest Nikon Ax confocal and guided tours of the LCI facility!

Join us IRL if you have registered or follow the talks on Zoom with this link (scientific program below).

You can also join us physically from 13:50 onwards for the Ax demo or the guided tour. Remember to book a time slot on the paper in front of the Erna Möller seminar hall in Neo.

9:00 – 9:05 Welcome (Staffan Strömblad)
9:05 – 9:15 Sylvie Le Guyader
Presentation of the Live Cell Imaging Core Facility
9:15 – 9:45 Christophe Leterrier, CNRS-Aix Marseille University, France
Looking at neurons at the nanoscale with super-resolution microscopy
9:45 – 09:50 Dusan Popov, European Product Manager Super Resolution, Nikon Europe B.V.
Future of superresolution imaging
9:50 – 10:05 Jianjiang Hu, Karolinska Institutet
Local temporal Rac1-GTP nadirs and peaks restrict cell protrusions and retractions
10:50 – 11:20 Joakim Lundeberg, The Royal Technology Institute, Stockholm, Sweden
Exploration of the transcriptome and genome in a tissue context
11:20 – 11:50 Guillaume Jacquemet, Åbo Akademi, Finland
Democratizing deep learning microscopy image analysis (ZeroCostDL4Mic)
11:50 – 11:55 Simone Lepper, European Product Manager Imaging Software & High- Content Screening, Nikon Europe B.V.
Future of Image Analysis
13:00 – 13:50 Featured presentation: Jennifer Lippincott-Schwartz, Senior Group Leader, Howard Hughes Medical Institute, Janelia Research Campus, USA
Emerging Imaging Technologies to Study Cell Architecture, Dynamics, and Function
13:50 –16:00 Open house at the Live Cell Imaging Core Facility / Tours / Demos

Brighter secondary antibodies

Fluorophores are in constant development.

There is now a new generation of Alexa Fluor fluorophores: the Alexa Fluor Plus. They can be purchased already coupled to secondary antibodies on the ThermoFisher website.

Brilliant violet antibodies are also very bright and despite their name, they come in all colours. The can be purchased from Biolegend which produces the fluorophore.

Another excellent source of bright fluorophores is the Janelia Fluor dyes. They can be purchased coupled to antibodies from the NovusBio website.

Jennifer Lippincott-Schwartz excellent talk on November 8th

Scilifelab invited Jennifer Lippincott-Schwartz to give a talk IRL in Solna on November 8th. Jennifer works a lot with microscopy and is an excellent speaker so her talk is guaranteed to be inspiring!

Here is more info.

2 Bioinformatician jobs at Scilife lab

The BioImage Informatics facility is our favorite image analysis facility at ScilifeLab! 🙂

They are looking for 2 bioinformatician working with microscopy images. Here is the first job ad. And here is the second one.

Please spread the word! 🙂

Online image analysis course

You can join the Switzerland’s Image and Data Analysis School, ZIDAS 2021.

This one-week school provides a hands-on introduction to image processing and analysis, with an emphasis on biologically relevant examples.

This school is for you if…

* you are a life-science researcher with a pressing need to quantify your light-microscopy images.

* you are uncertain about how to: Best calculate co-localisation, do deconvolution, automate the counting of cells, track objects over time, handle massive amounts of image data, record your image-analysis workflows in a reproducible manner.

More information can be found here: https://2021.zidas.org/

What can Artificial Intelligence do for you?

Check out the Nikon Artificial Intelligence (NIS.ai) webinar series to understand how Ai can help you in your microscopy experiments.

The NIS.ai Webinar Series will take place on Tuesdays at 14:00hrs and we are delighted to announce the first two talks:

June 29^th 14:00 – Dr Carlo Beretta from Heidelberg University on “How to build a Bioimage Analysis Workflow with multiple image analysis tools”
July 6^th14:00 – Dr Marko Popovic from Nikon Centre of Excellence in Amsterdam on “Quantitative pathology”

Program and free registration: here.

Artificial Intelligence tools

Artificial Intelligence, Deep Learning and Neuronal Networks are taking over the world, including microscopy. Within the next couple of years, you might well end up wondering how you got anything done without them!

Having an idea how Artificial Intelligence, Deep Learning and Neuronal networks work means that you will be able to come up with ideas about how they can help you in your research.

The Neubias (Network of European image bioanalysts) webinars and meetings are a good place to learn.

Here are 2 upcoming webinars about tools to train and use AI algorithms.

The first microscopy MOOC?

This MOOC for undergrad students by the University of Montpellier is about labeling and imaging vertebrate embryos.

All the videos are also available on their YouTube channel.

As far as I know, this is the first pure microscopy MOOC (Massive Open Online Course), dealing with sample preparation, imaging and data handling!

If you know of another microscopy MOOC (I know of some image analysis MOOC but not microscopy), please leave me a comment or sent me an email! 🙂

BII webinars and Global Bioimaging lectures

Lots of interesting courses and webinars about microscopy in these corona times. The BioImage Informatics facility at Scilife will present how useful it is to build imaging pipelines with Fiji/imageJ.

April 6th, 10:00-11:00: “ImageJ/Fiji – Make Your Own Macros – Overview”. This is not to teach how to script but to give you an overview of the scripting possibilities in ImageJ/Fiji. Please register here.

Version 2.0 of TissUUmaps is now released: TissUUmaps allows fast interactive display of tissue slide images and uses an overlay to display any sort of marker data on top. Be it spatially resolved gene expression, per cell data, or regions of interest. TissUUmaps is developed in the Wählby-Lab, with involvement of BIIF, and was first published in https://doi.org/10.1093/bioinformatics/btaa541.
Try out TissUUmaps and interact with a in-situ-sequencing dataset on a brain slice!

Advanced Methods in Bioimage Analysis, Online EMBO Practical Course, 26 Jun – 2 Jul 2021; Registration Deadline: 5 Apr 2021
This advanced course concentrates on teaching cutting-edge concepts and tools for quantitative image analysis, and will seek to upgrade the competencies of future bioimage analysis experts on both theoretical algorithm advancements as well as on practical implementation skills. BIIF is part of the scientific organization team. Register here.

Global BioImaging-ZEISS webinar series in Light Microscopy

Check here to see some nice general microscopy webinars by Global Bioimaging, the global pendant to Euro Bioimaging:

Tuesday, 30th March at 15:00 CEST: Introduction to Confocal Microscopy.
Tuesday, 20th April at 15:00 CEST: Multi-colour imaging – where are the limits & what are the opportunities?
Tuesday, 11th May at 15:00 CEST: Super-resolution: What is it? How can you do it? What does it enable?
TBA: From 2D to 4D imaging – how switching to a sheet of light has revolutionised volume acquisitions in Life Science.
TBA: Methods for Clearing your specimens – what are the options and which should you choose?
TBA: Automation in modern microscopy: easy ways to prevent costly accidents and enable your users with tools for easy experimental set up.

The LCI microscopy course 2021 starts next week! :)

As usual the lectures at the LCI microscopy course will broadcasted live online, free of charge and there is no need to register.

Title: Microscopy: improve your imaging skills – from sample preparation to image analysis

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

Applications are closed but all lectures will be broadcasted live and open to anyone without registration.

The course covers the following topics:

Optics, image formation, fluorescence, fluorophores, microscope and microscopy types
Objectives and refraction index, Cameras and detectors
Noise and background, Cameras and detectors, Bit depth and saturation, Multicolour imaging
Resolution and contrast, Sample preparation, Immunostaining
Nyquist sampling, Confocal and wide field settings, Scaling up and speeding up, High throughput/content
Volume imaging, deconvolution, multiphoton, Clearing and expansion
Live cell imaging, Fourier, AI, Super Resolution microscopy
Data handling, OMERO.figure, Requirements for image analysis, Colocalization
Image processing and analysis

Check the course schedule and details of how to join the Zoom webinars. Scroll down to read the kind testimonies of our dear students! 😊

Here is the course syllabus.

Hope you enjoy the LCI facility microscopy course 2021!