MicroscopyKarolinska – Page 9 – The Live Cell Imaging facility

Loads of microscopy jobs in Europe and even one at KI! :)

Good luck everyone who applies!

1- microscopy postdoc job at KI

I want to draw your attention to a current announcement from us about microscopy oriented post-doc positions. See more details here.

We are looking for qualified post-doc/s (1 or 2) for both:

* Technically-oriented tasks. Mainly related to fast fluorescence imaging techniques for fast 3D imaging in living animals.

* Data processing tasks. Mainly related to advanced image processing of data from fast 3D imaging in living animals.

Martin Köhler, PhD

The Rolf Luft Research Center for Diabetes and Endocrinology

2- job in a company in Switzerland

We at Nanolive have a new job posting for an R&D Biologist/Image Analysis expert based out of our offices on Lake Geneva, Switzerland. Do you enjoy creating custom macros or scripts and have experience with ImageJ and Cell Profiler? Are you an expert in Live Cell Imaging and looking for a new challenge with a cutting edge technique?

Nanolive, a growing scale-up company situated at EPFL Innovation Park but moving soon to the Lake Geneva Park in Tolochenaz, has developed a disruptive technology that, for the first time ever, allows users to explore a living cell in 3D without damaging it. To support its growth, Nanolive is looking for strengthening its application portfolio and its microscopes usability with a highly motivated and outstanding Biologist with experience in ImageJ and CellProfiler.

What we are looking for:

This position is ideal for a self-motivated, enthusiastic, independent, result oriented individual. Enjoy the benefits of working in a fast-growing start-up company where your efforts play a key role in the quality and success of the 3D Cell Explorer (CX).

If so, please apply here.

Paul Carman, Nanolive SA

3- A rare job as the manager of the excellent Nikon Imaging Center in London

We are hiring a new manager for the Nikon Imaging Centre@King’s College London. Further information is below and application info is here.

Nikon Imaging Centre Manager

Research Platforms, within the Research Management & Innovation Directorate at King’s College London is seeking to appoint a full-time staff member to manage the Nikon Imaging Centre (NIC).

The NIC is a world-class light microscopy facility on Guy’s Hospital Campus of King’s College London. The NIC houses a large number of state-of-the-art imaging equipment including confocal, super-resolution and multiphoton microscopes. The role holder will be responsible for the management, support and future development of the NIC with ongoing training and consultation with both King’s College London and Nikon staff. This includes providing expert guidance in experimental design and implementation, training for the users of the NIC and managing other NIC staff members.

There will also be opportunities for the post holder to contribute actively to research both individually and in collaboration with academic staff. We are looking for scientists with a special interest in the field of application of microscopy to study biomedicine. A PhD in the field of cell biology and/or microscopy, and extensive experience in the use of multiple forms of imaging methods and image analysis are essential. Previous experience of working within a core microscopy facility is also highly desirable.

This post will be offered on an indefinite contract.This is a full-time post – 100% full time equivalent.The selection process will include a panel interview.

Grade and Salary : The salary will be paid at Grade 7, £46,292 to £54,534 per annum inclusive of London Allowance. Job ID : 023306

Post Date : 08-Nov-2019 Close Date : 01-Dec-2019

Professor Maddy Parsons: maddy.parsons@kcl.ac.uk

4- and a microscopy specialist in Berlin

Light microscopy specialist, Advanced Light Microscopy Technology Platform, MDC; Berlin, Germany

A microscopy job in Portugal

This is an ad to a job as facility manager in Braga, Portugal. 🙂

Seminar on how to directly label your primary antibodies: Skip the secondary, part 3!

The LCI will host a seminar about GlyCLICK, a new way to directly label primary antibodies and stop using secondaries: 6th of November at 13:00 in the Lipid seminar room in Neo, KI Flemingsberg. The seminar will be broadcasted live. Here is how to find us and here how to follow the seminar online.

Everyone agrees that it would be great to be able to label our samples without using secondary antibodies.

less animals killed
shorter and cheaper protocols
no problem with isotype cross-reaction
no problem with secondary species when using many antibodies at once

There are many kits to label primary antibodies directly with fluorophores. The main disadvantage compared to primary/secondary stainings is that direct labels are often weaker because the final primary/fluorophore ratio is too low. Using an amplification method like TSA (Tyramide Signal Amplification) leads to a loss of resolution.

Over the years users at the LCI have tried this kit, this kit and this one. They gave mixed results depending on the antibody but we keep looking! Come to the LCI seminar about a new direct labelling technique that uses Click chemistry.

Apply now to the LCI microscopy course 2020 :)

It is now time to register to the LCI intensive microscopy course (Jan/Feb 2020). Check out the course schedule.

Loads of fun workshops, informative lectures, intense discussions and our popular Student Imaging Challenge workshop where students get direct feedback on how to improve their own sample preparation/experimental design.

We always run two courses in parallel:

the full course (#2870, 6 points, apply here) where students attend all activities
the theory only course (#2871, 4.5 points, apply here) for students who only attend the lectures

As usual, all lectures are public and broadcasted live so you are welcome to just show up (how to find us) or watch remotely (how to connect) without registration. Check the program as it may be updated in case of (unlikely) last minute changes.

We welcome your feedback about the quality of the webinar and the content of the lectures.

How to precisely measure the volume of a cell?

Measuring the volume of a cell is often done by labelling the cell membrane or its cytoplasm. Analysing large flat cells this way is easy but it is much harder for tiny cells like blood cells, yeast or bacteria.

Another way to measure volumes is to use a negative stain, i.e. where the medium is made fluorescent with a dye that does not go into the cell. The cell appears as a black hole in fluorescent images and unlike lipid-based membrane labelling, borders are even and easy to segment.

While many dyes can be used for live cells, one must choose large dyes when negatively imaging cells that have been fixed and permeabilized.

This paper and this one use high molecular weight (2000 KDa) Dextran to achieve these results and measure the size of bacteria.

This recent paper optimizes the technique.

Free genetic and image analysis tools

Here are a few image analysis tools (constructs for FRET, software…) that might be useful to you.

Construct for fluorescence biosensors and optogenetic tools
Free Image analysis software

http://www.hahnlab.com/tools/index.html

Free Image analysis software

https://www.utsouthwestern.edu/labs/danuser/software/

Free Image analysis software

http://cismm.web.unc.edu/

Party time at the LCI! :D

On the 10^th of October, the Live Cell Imaging facility will have an open house and a little party to celebrate loads of great stuff:

The LCI turned 10 years old this year! 😊
We got a wonderful light sheet system earlier this year. It is high time to give it a name and splash it with a bit of champagne!
Gisele Miranda from Scilife/BII has joined the LCI team to help our users with image analysis
We got a great server/analysis capacity set up by the KI IT department

Wow! What a year! 😃

Please come and celebrate with us! If you do not know what our microscopy facility has to offer, it is time to be curious and pay us a visit.

Open house: drop in between 9:00-11:00
Baptising of the light sheet system and celebration of Gisele and our shiny new server: at 11:00

All this will happen on the 10^th of October (next Thursday) at the LCI facility, on the 7^th floor of Neo at KI Flemingsberg. Here you can see how to find us.

Please help us spread the news! 😊

Deep red fluorescent proteins

The microscopy field is moving away from blue dyes. This is because red light, used to excited far red and deep red fluorophores, is less damaging to live cells than near UV light which is used to excite blue fluorophores.

On top of that, red light penetrates deeper into thick samples.

So as the trend in microscopy is to move to thicker samples and use more live samples, far red and deep red fluorophores are becoming more attractive.

Here is an article describing 3 new fluorescent protein in the far red to deep red range. One can excite them with 640 nm or a 685 nm lasers or LEDs.