January 2025 – The Live Cell Imaging facility

Clearing live samples!

A couple of super cool papers about transiently clearing live samples to obtain better images!

On Bioarxiv

In Vivo Optical Clearing of Mammalian Brain, Franzesi et al
Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo, Inagaki et al

In Science

Achieving optical transparency in live animals with absorbing molecules

The online LCI microscopy course: Microscopy: improve your imaging skills – from sample preparation to image analysis.

Dear all

It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28^th of Jan) and runs until the 13^th of February.

As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

Here is a selection of what we will talk about:

Optics and image formation,
How microscopes work
Objectives and refraction index
Cameras and detectors, Noise and background, Bit depth and saturation
Sample preparation, Immunostaining, Clearing
Resolution and contrast, Nyquist sampling, how to choose microscope settings
Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
Image processing and analysis

Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.

Scroll down to read the kind testimonies of our dear students! 🙂

We hope that you will enjoy the LCI facility online microscopy course!

Kindly forward to anyone who might be interested.

The LCI team

Correlative microscopy webinar

For those of you who are interested in Correlative microscopy where one overlays the data acquired from different imaging modalities, Zeiss organises a webinar about combining light microscopy, EM microscopy and Z ray data for very large samples.

Here is the link.