Dear all
If you like intravital microscopy and are looking for a job in Stockholm, check out this job ad at Stockholm University!
Month: March 2020
If you know anyone who is big in imaging and deserve the Lennart Nilsson Award have a look below!
Invitation to nominate candidates for the Lennart Nilsson Award 2020
Karolinska Institutet hereby invites nominations for the Lennart Nilsson Award 2020.
The Lennart Nilsson foundation was established in 1998 in order to bestow an award in recognition of the world-renowned Swedish photographer Lennart Nilsson and his extraordinary body of work. Its main aim is to promote education, training and research within the medical, biological and engineering sciences through the use of images.
This is achieved through the Lennart Nilsson Award, an international award bestowed annually upon an individual in recognition of outstanding contributions within the realm of scientific photography.
The nominees should fulfil the following criteria:
- Work in the spirit of Lennart Nilsson
- Make the invisible visible
- Reveal sciences to the world in beautiful, unique and powerful ways
- Visualize a scientific breakthrough
- Image reality in a surprising way
You are invited to nominate candidates to the Lennart Nilsson Award. Candidates should be active mainly in the Life Sciences and use pictorial representation as an explanatory medium. Users of animation technology are also eligible. The enclosed nomination form should be filled out and sent in together with the candidate’s CV, bibliography, articles, technical descriptions and pictorial material. Up to three letters with references and comments from experts may be included. Please attach the material as one PDF-file if possible and send to fonder@ki.se no later than Tuesday April 28, 2020. If you need to submit a larger file, you can send an e-mail to us for assistance.
The awarded amount is SEK 100 000 and the prize ceremony will take place in connection with the installation ceremony for new professors at Karolinska Institutet, Stockholm, Sweden October 15th.
More information about the Award can be found on http://ki.se/en/about/the-lennart-nilsson-award
For further questions please contact fonder@ki.se
One-step non-toxic clearing protocol
Do you know that clearing is not just about light sheet microscopy? Even if you have done your job well and your sample is directly on the coverslip (not on the slide), as soon as your sample is thicker than 10 um (1 cell diameter), you will see the effect of the refraction index mismatch.
What is that? Your sample and the mounting medium around it have a certain refraction index (or likely several). The objective you are using is designed for a certain refraction index (e.g. air, water or oil). If these refraction indices do not match what happens? as soon as you image a tiny bit away from the coverslip, the sample will look elongated, the intensity and contrast will drop very fast.
Sounds familiar? If yes, changing your mounting medium to match the objective will solve the problem. It works for light sheet but it also works for wide field or confocal imaging! Just change your mounting medium and you will see an enormous difference!
Here is an article describing a one-step clearing protocol. This basically is about using a different mounting medium. Easy, cheap and non-toxic! Give it a try!
Have a look at this post for more info.