Nice speaker line up at this 1 day event on the 18th of october.
Organized by Astra Zeneca, EMBL and several Swedish universities, this is a good mingling event for microscopists! 😀
Month: September 2019
Microscopy job in beautiful Singapore!
Anyone feeling like working in Singapore? Here is your chance! 😁
Crest V3 spinning disk confocal demo
Tomorrow (17 sept) we will enjoy a seminar and a live demo about the Crest V3 spinning disk confocal which is being set up at our facility as I write! 😀
Very cool confocal!
- enormous field of view (32 mm diameter)
- fully confocal
- can image at 100 frames per sec
- spits out Nyquist resolution with the 60x objective!
You can come to the seminar (at 10 in the Gene seminar room at the LCI facility) or listen to it remotely (see here how to follow the LCI webinars).
You can even book a private demo to image your own samples.
Matching the refraction index of live samples
To image a thick sample, it is crucial to match the refraction index of the sample with that of the immersion medium between the sample and the objective. Typically, life samples are in an aqueous solution like culture medium which has a refraction index of 1.33. Unfortunately organoids often have a higher refraction index closer to 1.44 therefore as one images deeper into the organoids, light scatters due to the refraction index mismatch and the images become blurry.
This paper presents a product that has a high RI and is compatible with cell culture. Good to keep in mind for those who image organoids over time.
How to identify cells and nuclei in an image?
NucleAlzer is a great new deep learning tool to identify roundish objects like nuclei and cells in fluorescent or bright field images.
To test if the tool works for you before you download it, you can simply upload one of your images and check the result. Easy! 😀