March 2018 – The Live Cell Imaging facility

Super resolution morning

On Thursday (15th of March), Teng-Leong Chew, director of the Advanced Imaging Center at the Janelia Research Center (Virginia, USA), will come to Stockholm and present what his facility can potentially do for you.

The AIC offers a crazy service where visiting scientists can use the super resolution systems they develop there with the help of their experts. This service is free of charge, including accommodation.

This can allow you to quickly run a project involving STED, PALM/STORM, SIM, adaptive optics or super resolution lattice light sheet microscopy.

Together with Leong’s presentation of his facility, there will be a few seminars by the Live Cell Imaging facility as well as several parts of the Advanced Light Microscopy facility at Scilife.

This is a great opportunity to catch up with what is available to you here and in the US. Hope to see you there! 🙂

Gluing samples

If your sample floats in its dish and you are desperate to image it, here is a good tip:

Vetbond is a good place to start! Glues super fast and is compatible with live cell imaging! 🙂

There are plenty of other similar products so please leave a comment to tell us if this works for you or not or which is your favorite tissue glue!

Skip the secondary! :)

Kromnigon makes fluorescent labels called FlexiStain that stick to biotin-labelled primary antibodies.

This means that you skip the species matching headache when using multiple antibodies and your labeling is faster! I have not tried myself but they offer free trial kits so give it a try! You are welcome to leave comments on this posts to tell us if it works or not! 🙂

Note: We have not had very good experience with these dyes. Check this post instead.