If you are tired of DAPI bleeding through your weak green channel but are stuck to using blue for the nucleus, you might want to give a try to Syto41.
Must better excitation efficiency at 405 nm and much narrower emission spectrum!
And Syto also comes in other colors. 🙂
Syto (and DAPI) is great but if you prefer to work on living cells one solution is to forgo fluorescence and instead use cells physical property of refractive index which is perfect for digital nuclear staining ( see http://nanolive.ch/cx/)
Are these phase objectives?Compatibility with fluorescence?
Hello they are compatible with fluorescence lots of details here including objectives http://nanolive.ch/fluo/ . thanks.