The Live Cell Imaging facility
@ Karolinska Institutet, Sweden
This course about light microscopy is made of videos only! It is very complete, very interesting and very well done!
Point Spread Function? Fourier transform? Image formation? Resolution? Everything about light microscopy you wish you had learned earlier! 🙂
Do your bit as a microscopy geek and download a great app called Resolution!
You can enter your objective of choice (magnification and NA) and get for the wavelength of your choice loads of useful information like the resolution in xy and z the optional sampling step…
But if you REALLY want to enter thegeek club, you must also get the Fourier Filter app! Hours of geeking fun ahead of you! 😉
Last call! Deadline 30th April!
Send your entries to the Nikon Small World image competition! Really nice prizes to be won including a trip to the Nikon headquarters in Japan! Many of the images produced daily at our facility are at the level of what is in their gallery so grab the chance! 🙂
Did you know about the BioImage Informatics Facility at Scilife? This is a great facility for image analysis. You can get 20h of expert help for free? Contact Petter Ranefall to get help with your analysis!
Several postdoctoral positions are opened at the Institute Pasteur (Paris, France) and at Princeton University (Princeton, NJ, USA) to visualize the topological and functional dynamics of small regulatory pieces of DNA, called enhancers, in the animal genome. Successful candidates will join a collaborative and interdisciplinary venture between the research groups of biophysicist Thomas Gregor and various collaborators at both institutes.
GOAL:
The dynamic organization of the genome in time and space plays a crucial role in the functional specification of a cell. In particular the interplay between multiple distant enhancers and their target gene promoters has critical mechanistic consequences on gene activity patterns during cell differentiation and development. We are developing state-of-the-art high-resolution live imaging techniques to resolve multiple enhancers in space and time to correlate the 3D motion of the DNA polymer with gene activity. The challenge is to develop the right imaging modalities that optimize our need for high temporal and spatial resolution, and to image a large field of view with multiple (>4) colors simultaneously.
PROFILE:
Candidates will have a strong interest for collaborative and interdisciplinary research. They should have a proven successful track record equipped with one, but ideally a combination of the following skills:
*Â Â Â Â Â Â Â Â live-cell microscopy, single molecule imaging
*Â Â Â Â Â Â Â Â microscope design and implementation
*Â Â Â Â Â Â Â Â hard- and software design for microscope control
*Â Â Â Â Â Â Â Â computational image analysis
Ability to work in collaboration with members of the lab and international collaborators in a dynamic, diverse and multinational group is essential. English is the working language.
Inquiries regarding the position and specific projects should be addressed to Thomas Gregor (tg2@princeton.edu<mailto:tg2@princeton.edu> ). Applications should include a statement of research interests and motivation, a CV, and contact information for three references. Applications will be reviewed as soon as they are received. Funding is available for multiple positions but candidates will be encourage to apply for independent competitive grants.
Here is our winner for the very first LCI Image of the week! Karl Annusver’s mouse skin sections labelled with Tomato (red) and AF488 (green). The other channels are label free imaging of fibers like collagen that either autofluoresce at the wavelengths used (690 and 780 nm) or give very nice Second Harmonic Generation. This allows us to visualize the unlabelled tissue surrounding the labelled cells.
Thanks Karl! 🙂
