January 2017 – The Live Cell Imaging facility

The LCI newsletter goes socially medial! :)

On top of its shiny new MicroscopyKarolinska.se blog, the LCI facility now has an LCI Facility Facebook account with a Microscopy@KI group.

Microscopy@KI is a public group so anyone with a Fb account can participate. Please use it as a light microscopy forum and post any light microscopy related question/answer, suggestion, announcement… The idea is for us all microscopy fans to share with each other loads of cool microscopy tips! B-)

And don’t forget to tell us at the LCI about the light microscope events you organize, cool new products for sample preparation you tried… so we can put them up on our blog and on Facebook!

LCI microscopy course 2017 is here!

The Live Cell Imaging facility will soon run its intensive microscopy course 17th Jan-9th Feb. Loads of fun workshops, informative lectures, intense discussions and our popular Student Imaging Challenge workshop where students get direct feedback on how to improve their own sample preparation/experimental design.

The full course (#2870) is full but the theory only course (#2871, 4.5 points) has unlimited space so you can still apply (mail me) until the 13^th.

As usual, all lectures are public so you are welcome to just show up without registration. The full program is on our our course webpage and instructions on how to find the Green seminar room can be found here. The program will be kept updated in case of (unlikely) last minute changes.

Additionally this year, we will do our best to broadcast all the lectures so that you do not need to physically come to Flemingsberg to listen to your favourite topic. Instead, just follow check the schedule on our course webpage, find out which lecture you want to follow and follow the LCI webinar link. We welcome your feedback about the quality of the webinar and the content of the lectures.

Try your sample on the Leica light sheet microscope!

We are delighted to host the Leica light sheet system, 25-26^th January!

Have a look at the cool videos they have on their website! Check here for details on how to:

Come and listen to the Leica Lightsheet seminar January 25^th 11:00-12:00, Red seminar room (how to find)
Book a private demo to image your own sample with the Leica specialist (January 25^th and 26^th).

Print your own hardware at KTH :)

Are you desperately looking for a small replacement part for some machine? Or as we did, a custom-made stage insert for our microscopes to accommodate 4 slides?

KTH has a very good 3D printing machine and Peter Arfert peter.arfert-at-sth.kth.se will work it all out in a blink!

Primo is a cool micropatterning device by Alveole

Primo is a cool micropatterning device that allows you to print your favourite protein on a Petri dish or a multi well plate with the pattern you want (down to 1 um resolution!). Print up to 3 proteins either in distinct or overlapping patterns, make gradients or even print on the walls of the wells. All you need is a jpeg image of your pattern! Then you can study how your favourite cells interact with the printed protein. See here how to book a time on the 18^th of January to see it action!

BioPen is a microfluidics device by FluiCell

One can pipette out a controlled concentration of a reagent onto one or a few cells inside a dish, without affecting the other cells. All this is done on the microscope so you can record the cell reaction and get quantitative data.The adjacent cells can serve as internal controls or can be used for repeats of the experiment. The device also has many channels, allowing you rinse the cell after exposure then expose it to other reagents and even mix the reagents in the different channels as you want. Check here to get to the webinar or see how to try BioPen with your own sample!

Welcome to the Live Cell Imaging facility @ Karolinska Institutet!

Here we are! A new blog for the LCI facility! 🙂

News, tips, tricks, thoughts… all about light microscopy. Please contribute, leave comments, send suggestions and click on that tempting Follow us button! 🙂