Imaris is a great image analysis software that is available to all the members of the Live Cell Imaging facility.
It is as easy to analyse 2D and 3D image files with Imaris. The software also allows you to make great multidimensional plots to present your data.
One can count objects inside objects (example number of vesicles per cell), measure shortest distances from one type of object to another (example distance from vesicles to the cell membrane), track cells even when they divide, trace neurons or blood vessels… all this in 3D, time, several colours.
On the 22nd of May, the LCI will host an Imaris workshop.
The morning seminar (held in Neo/DNA room) from 10-12 will be broadcasted for those who cannot join. Please follow the instructions on our website to follow the webinar.
In the afternoon, we will analyse the data of our users. Submit your images to DONTCHEVA Guergana (g.dontcheva(at)bitplane.com).
Do not miss the seminar tomorrow morning at Scilife: the inventor of the CUBIC technique, Etsuo Susaki, will present his technique which can clear fatty and dense tissues.
Click here to know where and when.
And here is the link to the updated Cubic protocol.
The Live Cell Imaging facility is back with its intensive microscopy course! In 2018 we moved to a new building so there was no course but we will strike again in Jan-Feb 2019!!You will definitely learn tons at our course. Have a look at the program and judge for yourself.
As usual we run 2 courses in parallel:
#2870, 6 points, is the full course with all lectures, workshops, demos… This will run 22/jan-08 feb, 3 days/week 9:00-17:15.
#2871, 4.5 points, is the same minus some workshops and demos. This course will run at the same dates but 10:00-15:00.
The rest of the time is used for home assignments.
All lectures are open to the public without any registration so tell your colleagues!
Course applications are open from today and until the 15th of November. The full course (2870) has only 16 spots available so just go for it NOW! 🙂
Expansion microscopy and clearing are fantastic tools for anyone who images thick (> 1 cell diameter) fixed samples. We are now lucky to have access to state of the art talks and hands-on workshops at Scilife in September. Register early not to be disappointed!
Please apply to Hans (first day) and David (second day) directly!
If you are looking for an inspiring talk about a super freeware for image analysis, go to Scilife in Uppsala on the 8th of June and listen to Anne Carpenter from the Broad Institute where CellProfiler was born! Check out the details here.
Next wednesday morning, David Unnersjö-Jess from KTH/Scilife will give a talk at the Live cell imaging facility (9:30, DNA seminar room), about a new and exciting aspect of microscopy: Expansion microscopy.
This is a technique where one ‘blows up’ the sample while keeping all proteins in place and at the same relative distance from each other. The sample is simply ‘inflated’. One can then take images of it with a normal microscope but the resulting image give a much higher resolution than normal microscopy.
After the seminar, David will have an open discussion with anyone who would like to try the technique.
On Thursday (15th of March), Teng-Leong Chew, director of the Advanced Imaging Center at the Janelia Research Center (Virginia, USA), will come to Stockholm and present what his facility can potentially do for you.
The AIC offers a crazy service where visiting scientists can use the super resolution systems they develop there with the help of their experts. This service is free of charge, including accommodation.
This can allow you to quickly run a project involving STED, PALM/STORM, SIM, adaptive optics or super resolution lattice light sheet microscopy.
Together with Leong’s presentation of his facility, there will be a few seminars by the Live Cell Imaging facility as well as several parts of the Advanced Light Microscopy facility at Scilife.
This is a great opportunity to catch up with what is available to you here and in the US. Hope to see you there! 🙂
Super resolution course early February at Scilife with great presentations accessible to everyone! I will go to the DNA-paint talk! 🙂
Check it out here.
Interesting seminars in Uppsala. Apparently this endoscope can go inside our bodies and has has tiny objective of only 300um!
They will also present at KI (if you are interested contact Gilles Cestelli gilles(at)maunakeatech(dot)com)
Great news! Xiaowei Zhuang from Harvard University will visit us on the 31st of May!
On top of the STORM super resolution technique, her lab recently came up the MERFISH technique -a multiplexing-based in situ RNA sequencing-, as well as a FRET-based method to study Protein-DNA transient interactions during replication, transcription…
Check here her projects and mind-blowing ‘selected’ publication list.
Come and listen to her talk on Wednesday, May 31 at 10.00-11.00. Main lecture Hall 4th Floor, Novum, Flemingsberg
”Illuminating biology at the nanoscale and systems scale using single-molecule and super-resolution imaging”.
Hope to see you there! 🙂