Hardware – Page 2 – The Live Cell Imaging facility

The LCI microscopy course 2022 is now open for registrations!

It is now time to apply to the intensive LCI microscopy course Jan/Feb 2022: Microscopy: improve your imaging skills – from sample preparation to image analysis

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that their knowledge is limited.

All the lectures at the LCI microscopy course will also broadcasted live online, free of charge and there is no need to register.

All details about the course including course schedule, how to apply, and how to follow the lectures are found here.

Scroll down to read the kind testimonies of our dear students! 😊

Hope you enjoy the LCI facility microscopy course 2022!

Tweety turned into a power machine!

Our dear Tweety microscope, which was simplest and cutest of all the LCI systems, has muted into our most sophisticated power machine!

On the 24^th at 10, we will run an online demo (link below) to show what our upgraded Tweety can deliver:

Much larger field of view (from 18 mm diagonal to 25 mm)
Upgraded single point confocal on the left side
Resonant scanner with 1024×1024 pixels (compared to 512×512 on our other resonant scanners), still the same speed (30 fps) and improved low noise
Spinning disk confocal on the right side with bypass to image wide field
2 very sensitive cameras on the right side: one with the very large field of view and 11um pixels for best sensitivity and one with the normal field of view and 6.45 pixels for best resolution
Our great Primo is still on the back of the microscope to allow micropatterning of proteins at the bottom of a dish or micromanufacturing of wells in the shape/pattern of your choice
2 wonderful silicon immersion objectives specialized for tissue imaging with automatic correction ring: 20x/1.05 and 40x/1.25

After the demo, the LCI users who have already been trained on our widefield systems can get access to Tweety for free after a mandatory short training.

Please add the demo in your calendar and make sure to test the link ahead of the meeting.

Link to the Zoom Meeting on the 24^th at 10am: https://ki-se.zoom.us/j/7302561100

Super-Resolution spinning disk demo at the LCI!

Dear microscope freaks

How would you like to run some gentle live sample imaging with a 60x objective with:

an xy resolution of 120 nm without software tricks (or even better after deconvolution),
the great contrast of a true confocal,
82 frames per second,
or decide to bypass everything, go widefield and image at 100 frames per second with a super large field of view (220×220 um)?

Sounds good to me! 🙂

For the next 2 weeks you can do that with the new toy on demo at the LCI facility!

The beast is a new sort of spinning disk confocal and is called SoRa (Super-Resolution Optical Reassignment). It is a collaboration between Nikon and Yokogawa.

We even have 2 cameras to compare (Prime95B and BSI from Photometrics).

Oliver Garner from Bergman Labora will give a short online presentation of how SoRa works on Monday (29^th) at 13:00. The presentation is done remotely and broadcasted live. You can join the audience from the comfort of your office chair by following the instructions here (please try beforehand to make sure all works).

Interested in trying it? Please contact us.

A dream!!

Great video tutorials for our Primo users and paper on microniches

Hello again

Here you can see very nice video tutorials on the Alveole website and this is a cool article by Viasnoff et al about making 3D microniches with 1 um resolution! And you can do this at the LCI facility!! 🙂

Confocal endoscopy talk in Uppsala

Interesting seminars in Uppsala. Apparently this endoscope can go inside our bodies and has has tiny objective of only 300um!

They will also present at KI (if you are interested contact Gilles Cestelli gilles(at)maunakeatech(dot)com)

Custom made rotating stage insert

One more cute stage insert for the LCI facility, thanks to Peter Arfert, the KTH workshop wizard and his 3D printing machine! 🙂

Try your sample on the Leica light sheet microscope!

We are delighted to host the Leica light sheet system, 25-26^th January!

Have a look at the cool videos they have on their website! Check here for details on how to:

Come and listen to the Leica Lightsheet seminar January 25^th 11:00-12:00, Red seminar room (how to find)
Book a private demo to image your own sample with the Leica specialist (January 25^th and 26^th).

Print your own hardware at KTH :)

Are you desperately looking for a small replacement part for some machine? Or as we did, a custom-made stage insert for our microscopes to accommodate 4 slides?

KTH has a very good 3D printing machine and Peter Arfert peter.arfert-at-sth.kth.se will work it all out in a blink!

Primo is a cool micropatterning device by Alveole

Primo is a cool micropatterning device that allows you to print your favourite protein on a Petri dish or a multi well plate with the pattern you want (down to 1 um resolution!). Print up to 3 proteins either in distinct or overlapping patterns, make gradients or even print on the walls of the wells. All you need is a jpeg image of your pattern! Then you can study how your favourite cells interact with the printed protein. See here how to book a time on the 18^th of January to see it action!

BioPen is a microfluidics device by FluiCell

One can pipette out a controlled concentration of a reagent onto one or a few cells inside a dish, without affecting the other cells. All this is done on the microscope so you can record the cell reaction and get quantitative data.The adjacent cells can serve as internal controls or can be used for repeats of the experiment. The device also has many channels, allowing you rinse the cell after exposure then expose it to other reagents and even mix the reagents in the different channels as you want. Check here to get to the webinar or see how to try BioPen with your own sample!