Nov 30th-Dec 2nd, Janelia Research Center organizes a great image analysis workshop with loads of goodies. Everything is online and free. There are in depth and more general workshops about Deep Learning with different freeware, programming and scripting of all sorts, Big data things… Check the program here.
Tag: Cool talk
Super resolution course and webinars at ALM
Dear all
See here for more details about the ALM super resolution microscopy course in January. Note that the webinars are open to everyone (see registration email at the bottom of the page) and are always very interesting talks. 😊
Freeware to turn widefield, TIRF or confocal images into super resolution images!
Check out this webinar tomorrow by Rickardo Henriques, the inventor of SRRF (surf), post processing magic for all types of images!
*Title:* Open and accessible cutting-edge technology for super-resolution and machine-learning enabled microscopy
*When: *June 5th 4:00 pm CEST
*How to access/register*
And here’s a small teaser if you want to quickly see the topics we’ll cover.
Great webinars about sample preparation at Scilife
Hans Blom from the Advanced Light Microscopy facility at Scilife organises next week (10th and 1tth of June) 3 sessions of great seminars:
- Sample preparation for super resolution. These seminars include the great technique of expansion where one can ‘image in super resolution’ without a super resolution microscope.
- Clearing with CUBIC. Great clearing technique that works for all organs. The authors clear whole adult mice!!!
- Light sheet microscopy by Bruker. Learn about the latest light sheet development.
I especially recommend the CUBIC seminar to anyone who wants to image samples thicker than 100 um. This will teach you how to treat your sample to make it fully transparent so you can image through many cm of tissue!
🙂
Neubias webinars on Youtube
Updates on the next events of the NEUBIAS Academy@Home Webinar series,
Newly confirmed events:
5 May: ilastik beyond pixel classification, by Anna Kreshuk and Dominik Kutra-
6 May: GPU-Accelerated Image Processing with CLIJ2, by Robert Haase
7 May: Interactive Bioimage Analysis with Python and Jupyter, by Guillaume Witz
Upcoming events open to registration:
LAST CHANCE TO REGISTER:
28 April: Introduction to nuclei segmentation with StarDist, by Martin Weigert et al
29 April: Quantitative Pathology and Bioimage Analysis: QuPath v0.2.0, By Pete Bankhead
30 April: Advanced Image Processing with MorphoLibJ, by David Legland
Two weeks after the opening of the Academy and of the registrations, Webinars and online courses have already attracted over 5,000 registrations!
The events are recorded and some are already available on the Youtube NEUBIAS Channel.
Furthermore, a thread will be opened in the image.sc Forum to report Q&As and to welcome further questions/comments for each event.
You’ll find more information here.
Neubias online school of image analysis
Neubias is back with new ideas! Neubias is the Network of European Bioimage Analysts and what they burn for is to help scientists analyze their images.
Possibly inspired by the Corona time, they will start an online school for image analysis based on video tutorials and online events.
Have a look at their new page called Neubias academy where they announce several events coming up in the next few months.
Great video Microcourses about microscopy
Jennifer Watson’s Microcourses channel on You Tube is really recommended to anyone who uses a microscope.
Short targetted videos that will boil down the principles of light microscopy for biologist and help you understand what you are doing.
There are a few series, each of them with a few videos and the collection is constantly growing. Remember to subscribe so you get to know when they post a new one.
Seminar on how to directly label your primary antibodies: Skip the secondary, part 3!
The LCI will host a seminar about GlyCLICK, a new way to directly label primary antibodies and stop using secondaries: 6th of November at 13:00 in the Lipid seminar room in Neo, KI Flemingsberg. The seminar will be broadcasted live. Here is how to find us and here how to follow the seminar online.
Everyone agrees that it would be great to be able to label our samples without using secondary antibodies.
- less animals killed
- shorter and cheaper protocols
- no problem with isotype cross-reaction
- no problem with secondary species when using many antibodies at once
There are many kits to label primary antibodies directly with fluorophores. The main disadvantage compared to primary/secondary stainings is that direct labels are often weaker because the final primary/fluorophore ratio is too low. Using an amplification method like TSA (Tyramide Signal Amplification) leads to a loss of resolution.
Over the years users at the LCI have tried this kit, this kit and this one. They gave mixed results depending on the antibody but we keep looking! Come to the LCI seminar about a new direct labelling technique that uses Click chemistry.
EMBL microscopy event in Göteborg
Nice speaker line up at this 1 day event on the 18th of october.
Organized by Astra Zeneca, EMBL and several Swedish universities, this is a good mingling event for microscopists! 😀
Crest V3 spinning disk confocal demo
Tomorrow (17 sept) we will enjoy a seminar and a live demo about the Crest V3 spinning disk confocal which is being set up at our facility as I write! 😀
Very cool confocal!
- enormous field of view (32 mm diameter)
- fully confocal
- can image at 100 frames per sec
- spits out Nyquist resolution with the 60x objective!
You can come to the seminar (at 10 in the Gene seminar room at the LCI facility) or listen to it remotely (see here how to follow the LCI webinars).
You can even book a private demo to image your own samples.