Brilliant violet dyes

Those who work with FACS might be aware of the very bright dyes called Brilliant Violet. They can also be used in microscopy. 🙂

BV dyes are all excited around 400 nm (same as blue dyes) but they can emit at much longer wavelengths, like red or far red. This means that if they are used together, they are excited at the same time and the colors are only separated based on the emission! But these dyes are very bright which is a great advantage.

Et voilà! 🙂

Easy label of live cells

How to easily label Golgi, cytoskeleton, endosomes, lysosomes… in live cells with CFP, GFP or RFP then be able to fix everything? It does sound like pure magic! Have a look here.

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