Just heard this amazing Eurobioimaging talk by Florian Shueder from EFPL. They published FLASH-PAINT, an improved version of DNA-Paint.
FLASH-PAINT combined with fluorogenic probes, eraser oligos and EXCHANGE-PAINT has many advantages over DNA-PAINT:
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- It keeps the very high resolution of just a few nm.
- It is very fast.
- It enables imaging with a widefield system instead of in TIRF.
- It enables high multiplexing (they demonstrate 12 rounds) without needing to wash.
They demonstrate 12-plex imaging with 25nm resolution in just 2h!
