One of the major pains with membrane dyes for live cells is that within 30min, all the vesicles inside the cells are also labelled. Another major pain is when you discover that your dye is not fixable…
The CellBright dyes label membrane is apparently fixable, even with methanol, and doesn’t do the flip flop thing! Potentially extremely useful for image analysis!
Please leave a comment to let us know how it went for you! 🙂
If you are looking for an inspiring talk about a super freeware for image analysis, go to Scilife in Uppsala on the 8th of June and listen to Anne Carpenter from the Broad Institute where CellProfiler was born! Check out the details here.
Here is where to register for the next Neubias, the European Image Analysis school! Learn how to build your own image analysis pipeline with expert help! 🙂
Only a few days left to register!
Neubias is a European effort to get biologists comfortable with image analysis. conferences (Hungary, Jan 2018), courses, facility staff training, ‘biologists meet analysts’ meetings… They have it all!
Now this is your chance to learn and get help to analyze images for your microscopy project! Check the Neubias conference and courses in Hungary!
And if you would like to work with microscopy in the future, remember to join the ELMI (European Light Microscopy Initiative) so you get all their job announcements! 🙂
Up to three postdoc and researcher positions in Computerized Image Processing, Scientific Computing, and Bioinformatics in Uppsala
Do you want to develop new, intelligent ways of processing and managing very large amounts of microscopy images in order to leverage the imminent explosion of information from modern experimental setups in the biosciences? Join the recently started interdisciplinary research project Hierarchical Analysis of Spatial and Temporal Data (HASTE) within the research groups of Prof. Carolina Wählby, Assoc. Prof. Andreas Hellander, Department of Information Technology, and Assoc. Prof. Ola Sjuth, Department of Pharmaceutical Biosciences, Uppsala University, Science for Life Laboratory (SciLifeLab), Vironova AB and AstraZeneca AB, Sweden.
More information and instructions for how to apply can be found here (Postdoc: If your PhD degree was obtained after 2014-05-23) or here (Researcher: If your PhD was obtained before 2014-05-23).
Deadline for application: 2017-05-23. Note that all applications need to be submitted though the online recruitment system.
Would you like to boost your knowledge about Imaris, learn loads of tipes and get expert help with your pipeline? Join the Imaris User Group Meeting in Basel at the end of June!