Good news on the glass bottom plates and chamber front!

There are loads of multiwell plates and chamber slides on the market but it is hard to get any that fulfill these criteria:

  • They do not kill the cells (unfortunately, this is not a joke!)
  • They do not leak (no joke either! Sigh…)
  • They have a low skirt: so they can be completely imaged (including outer part of outer wells) with our Nikon 20x air, NA 0.75, WD 1mm objectives. The ‘skirt’ is the rim at the edge of the plate. A high skirt raises the plate so that it becomes impossible to image the wells at the edges of the plate as the objective bumps into the insert edge.
  • They have a #1.5 glass bottom
  • They don’t cost a bomb.

We recently found a great source of plates and chambers that fulfill all these criteria and are very flat! Check the Zell Kontakt website (email under Legal disclaimer)! 🙂

Brilliant violet dyes

Those who work with FACS might be aware of the very bright dyes called Brilliant Violet. They can also be used in microscopy. 🙂

BV dyes are all excited around 400 nm (same as blue dyes) but they can emit at much longer wavelengths, like red or far red. This means that if they are used together, they are excited at the same time and the colors are only separated based on the emission! But these dyes are very bright which is a great advantage.

Et voilà! 🙂

Easy label of live cells

How to easily label Golgi, cytoskeleton, endosomes, lysosomes… in live cells with CFP, GFP or RFP then be able to fix everything? It does sound like pure magic! Have a look here.