Microscopy job in Manchester

Senior Experimental Officer (Bioimaging / Image Analysis) The University of Manchester, UK

The University of Manchester Bioimaging Core Facility is recruiting an image processing/analysis Senior Experimental Officer. This position is initially for 2 years, but with a view to becoming a permanent position. Manchester is a great place to live and work and you’ll be part of a team supporting over 20 microscope systems and 200 PIs. We run a mix of commercial, open source and bespoke software and the successful candidate would be responsible for developing, applying and teaching these tools to the Facility users. You don’t need to be from an academic background, we’d be very keen to recruit someone currently working within the commercial side of image analysis and a PhD is not essential – we’re looking for the right person not the right qualifications.

Due to the demand for advanced image processing and data analysis, a new position has been created to support the users of the Bioimaging Facility in the Faculty of Biology, Medicine and Health.

You will work as part of the Bioimaging team, led by Dr Peter March, but you will be responsible for developing the image processing capabilities of the Facility. Combining both commercial packages and your own bespoke scripted solutions you will provide analysis pipelines to support users who have acquired large multi-dimensional datasets using the Bioimaging Facility microscopes. You will have multiple projects to manage simultaneously and so excellent project management skills are also required.

You will have completed a PhD in science, mathematics, computer science or a relevant discipline, or equivalent experience. A knowledge of cell biology is not essential, but you must have proven experience in developing analysis pipelines and the ability to write custom scripts. Excellent communication and interpersonal skills are also required.

As an equal opportunities employer we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status.  All appointments are made on merit.

Please note that we are unable to respond to enquiries, accept CVs or applications from Recruitment Agencies.

Enquiries about the vacancy, shortlisting and interviews:

Name: Dr Peter March

Email: P.March@amcnhester.ac.uk Tel: 0044 161 275 1571 or 0044 7747 118 447

General enquiries:

Email: hrservices@manchester.ac.uk Tel: 0161 275 4499

Technical support:

Email: universityofmanchester@helpmeapply.co.uk Tel: 0161 850 2004

This vacancy will close for applications at midnight on the closing date.

Closing date: 30/01/2020

Salary: £41,526 to £51,034 per annum according to relevant experience.

Employment type: Fixed Term

Department: Biology, Medicine & Health

Hours per week: Full time

Contract Duration: From January 2020 until 31 December 2022 Job reference: BM&H-14959

Fluorophores that blink for STORM without any special buffer

Sounds good! No more buffer that stops working after one hour of imaging!

This company sells SaraFluor650 secondary antibodies. This fluorophore seems to be a natural blinker which does not need to reducing environment. Apparently it doesn’t need very bright lasers either!

Apparently they have also developed a green variant. So you can run 2-colour direct STORM on your favorite TIRF system! 😀

They also sell pH sensors and more so check their website and if you try, write a comment to let us know how things worked!

Microscopy facility job in Hungary

Dear Colleagues!

I would like to draw your attention to the open position at the Hungarian Center of Excellence for Molecular Medicine.

Best wishes,

Jozsef

———————————————————–

Advanced Core Facilities at HCEMM

High-quality molecular medicine-oriented research activities at the Hungarian Center of Excellence for Molecular Medicine (HCEMM) are supported by a matrix of advanced core facilities (ACFs). In the premises of the Hungarian Research Institutions, three ACFs are currently being set-up:

  • Functional Cell Biology and Immunology ACF (University of Szeged)
  • In vivo Imaging ACF (Semmelweis University, Budapest)
  • Single-Cell Omics ACF (Biological Research Center, Szeged)

The Call

The Project Steering Committee of the HCEMM project hereby announces a call for excellent, professional “Heads of Advanced Core Facilities”.

We are searching for Heads for each of the above ACFs who operate, manage, supervise, and develop the respective HCEMM AFC. We seek dynamic and interactive individuals with an excellent professional track record and demonstrated experience and interest in organizing and spearheading the ACF.

HCEMM is an inclusive, equal opportunity employer offering attractive working conditions and benefits appropriate to a molecular medicine research organization. Female candidates are strongly encouraged to apply.

An initial contract of 5 years will be offered to the successful candidates; this is foreseen to be extended to a maximum of 9 years, subject to an external, independent, international review. Successful candidates will receive an attractive remuneration package and an opportunity to recruit two technicians for each ACF. Importantly, the selected ACF Heads will be constantly helped and trained by heads and managers of the state-of-the-art system of Core Facilities at EMBL.

Technical notes

Please visit https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.hcemm.eu&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C096431407c66429c33c408d77ed8c1e5%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637117341763082188&sdata=AWRzMmDqkw%2B6Qtuctsvw1qmenkmrhiOUEoijTilFKFs%3D&reserved=0 for more details related to HCEMM (Synopsis of HCEMM), to the positions (Description of positions) and to the ACFs (Technical description of the ACFs) as well as the Evaluation process & Criteria.

Applicants should submit a cover letter, a CV, a detailed presentation of the professional experience, and a concise description of research interest and plans, all edited as a single PDF file, to career@hcemm.eu by January 17, 2020. Please also arrange for 3 letters of recommendation to be also emailed to career@hcemm.eu by the same deadline.

József Maléth M.D., Ph.D.

Principal Investigator, Research associate

MTA-SZTE Momentum Epithel Cell Signalling and Secretion Research Group University of Szeged, First Department of Medicine Koranyi alley 8-10, Szeged, H-6720, Hungary

Tel: +36-62-545-201

Cell: +36-70-4166500

E-mail: jozsefmaleth1@gmail.com;

maleth.jozsef@med.u-szeged.hu

Multiplexed immunostaining

It is not easy to find enough antibodies that work together to be able to label a sample with more than 4 antibodies at the same time. And even 4 is pushing it.

This paper describes a new immunostaining multiplexing method called 4i. The method is based on a special imaging buffer that prevents the antibody from being strongly bound to the sample due to the imaging process. This allows the authors to detach the antibody with gentle treatments, leaving the sample in a good shape and ready for another round of labeling and imaging.

Using this method they have successfully labelled the same sample with 40 different primary/secondary of the shelf antibody pairs!

Loads of microscopy jobs in Europe and even one at KI! :)

Good luck everyone who applies!

1- microscopy postdoc job at KI

I want to draw your attention to a current announcement from us about microscopy oriented post-doc positions. See more details here.

We are looking for qualified post-doc/s (1 or 2) for both:

* Technically-oriented tasks. Mainly related to fast fluorescence imaging techniques for fast 3D imaging in living animals.

* Data processing tasks. Mainly related to advanced image processing of data from fast 3D imaging in living animals.

Martin Köhler, PhD

The Rolf Luft Research Center for Diabetes and Endocrinology

2- job in a company in Switzerland

We at Nanolive have a new job posting for an R&D Biologist/Image Analysis expert based out of our offices on Lake Geneva, Switzerland.   Do you enjoy creating custom macros or scripts and have experience with ImageJ and Cell Profiler?  Are you an expert in Live Cell Imaging and looking for a new challenge with a cutting edge technique?

Nanolive, a growing scale-up company situated at EPFL Innovation Park but moving soon to the Lake Geneva Park in Tolochenaz, has developed a disruptive technology that, for the first time ever, allows users to explore a living cell in 3D without damaging it. To support its growth, Nanolive is looking for strengthening its application portfolio and its microscopes usability with a highly motivated and outstanding Biologist with experience in ImageJ and CellProfiler.

What we are looking for:

This position is ideal for a self-motivated, enthusiastic, independent, result oriented individual. Enjoy the benefits of working in a fast-growing start-up company where your efforts play a key role in the quality and success of the 3D Cell Explorer (CX).

If so, please apply here.

Paul Carman, Nanolive SA

3- A rare job as the manager of the excellent Nikon Imaging Center in London

We are hiring a new manager for the Nikon Imaging Centre@King’s College London. Further information is below and application info is here.

Nikon Imaging Centre Manager

Research Platforms, within the Research Management & Innovation Directorate at King’s College London is seeking to appoint a full-time staff member to manage the Nikon Imaging Centre (NIC).

The NIC is a world-class light microscopy facility on Guy’s Hospital Campus of King’s College London. The NIC houses a large number of state-of-the-art imaging equipment including confocal, super-resolution and multiphoton microscopes. The role holder will be responsible for the management, support and future development of the NIC with ongoing training and consultation with both King’s College London and Nikon staff. This includes providing expert guidance in experimental design and implementation, training for the users of the NIC and managing other NIC staff members.

There will also be opportunities for the post holder to contribute actively to research both individually and in collaboration with academic staff. We are looking for scientists with a special interest in the field of application of microscopy to study biomedicine. A PhD in the field of cell biology and/or microscopy, and extensive experience in the use of multiple forms of imaging methods and image analysis are essential. Previous experience of working within a core microscopy facility is also highly desirable.

This post will be offered on an indefinite contract.This is a full-time post – 100% full time equivalent.The selection process will include a panel interview.

Grade and Salary :            The salary will be paid at Grade 7, £46,292 to £54,534 per annum inclusive of London Allowance.          Job ID :  023306

Post Date :          08-Nov-2019      Close Date :        01-Dec-2019

Professor Maddy Parsons: maddy.parsons@kcl.ac.uk

4- and a microscopy specialist in Berlin

Light microscopy specialist, Advanced Light Microscopy Technology Platform, MDC; Berlin, Germany

 

Seminar on how to directly label your primary antibodies: Skip the secondary, part 3!

The LCI will host a seminar about GlyCLICK, a new way to directly label primary antibodies and stop using secondaries: 6th of November at 13:00 in the Lipid seminar room in Neo, KI Flemingsberg. The seminar will be broadcasted live. Here is how to find us and here how to follow the seminar online.

Everyone agrees that it would be great to be able to label our samples without using secondary antibodies.

  • less animals killed
  • shorter and cheaper protocols
  • no problem with isotype cross-reaction
  • no problem with secondary species when using many antibodies at once

There are many kits to label primary antibodies directly with fluorophores. The main disadvantage compared to primary/secondary stainings is that direct labels are often weaker because the final primary/fluorophore ratio is too low. Using an amplification method like TSA (Tyramide Signal Amplification) leads to a loss of resolution.

Over the years users at the LCI have tried this kit, this kit and this one. They gave mixed results depending on the antibody but we keep looking! Come to the LCI seminar about a new direct labelling technique that uses Click chemistry.

Supplies of Zell Kontakt products

Zell Kontakt is a German company making fantastic glass bottom dishes and plate. Check out why the LCI facility likes their products (absolutely no commercial interests!).

Great products but crap communication skills! Either their website is down or they do not answer the phone and emails and anyway if they do, make sure you have a German colleague around because no one speaks English.

Now we have found a way around the problem: MoBiTec sells Zell Kontakt products. 🙂

If you are at KI and need to find a KI-certified supplier, just come and ask us. 🙂

How to precisely measure the volume of a cell?

Measuring the volume of a cell is often done by labelling the cell membrane or its cytoplasm. Analysing large flat cells this way is easy but it is much harder for tiny cells like blood cells, yeast or bacteria.

Another way to measure volumes is to use a negative stain, i.e. where the medium is made fluorescent with a dye that does not go into the cell. The cell appears as a black hole in fluorescent images and unlike lipid-based membrane labelling, borders are even and easy to segment.

While many dyes can be used for live cells, one must choose large dyes when negatively imaging cells that have been fixed and permeabilized.

This paper and this one use high molecular weight (2000 KDa) Dextran to achieve these results and measure the size of bacteria.

This recent paper optimizes the technique.

 

Free genetic and image analysis tools

Here are a few image analysis tools (constructs for FRET, software…) that might be useful to you.

  • Construct for fluorescence biosensors and optogenetic tools
  • Free Image analysis software

http://www.hahnlab.com/tools/index.html

  • Free Image analysis software

https://www.utsouthwestern.edu/labs/danuser/software/

  • Free Image analysis software

http://cismm.web.unc.edu/