Microscopy job in Manchester

Senior Experimental Officer (Bioimaging / Image Analysis) The University of Manchester, UK

The University of Manchester Bioimaging Core Facility is recruiting an image processing/analysis Senior Experimental Officer. This position is initially for 2 years, but with a view to becoming a permanent position. Manchester is a great place to live and work and you’ll be part of a team supporting over 20 microscope systems and 200 PIs. We run a mix of commercial, open source and bespoke software and the successful candidate would be responsible for developing, applying and teaching these tools to the Facility users. You don’t need to be from an academic background, we’d be very keen to recruit someone currently working within the commercial side of image analysis and a PhD is not essential – we’re looking for the right person not the right qualifications.

Due to the demand for advanced image processing and data analysis, a new position has been created to support the users of the Bioimaging Facility in the Faculty of Biology, Medicine and Health.

You will work as part of the Bioimaging team, led by Dr Peter March, but you will be responsible for developing the image processing capabilities of the Facility. Combining both commercial packages and your own bespoke scripted solutions you will provide analysis pipelines to support users who have acquired large multi-dimensional datasets using the Bioimaging Facility microscopes. You will have multiple projects to manage simultaneously and so excellent project management skills are also required.

You will have completed a PhD in science, mathematics, computer science or a relevant discipline, or equivalent experience. A knowledge of cell biology is not essential, but you must have proven experience in developing analysis pipelines and the ability to write custom scripts. Excellent communication and interpersonal skills are also required.

As an equal opportunities employer we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status.  All appointments are made on merit.

Please note that we are unable to respond to enquiries, accept CVs or applications from Recruitment Agencies.

Enquiries about the vacancy, shortlisting and interviews:

Name: Dr Peter March

Email: P.March@amcnhester.ac.uk Tel: 0044 161 275 1571 or 0044 7747 118 447

General enquiries:

Email: hrservices@manchester.ac.uk Tel: 0161 275 4499

Technical support:

Email: universityofmanchester@helpmeapply.co.uk Tel: 0161 850 2004

This vacancy will close for applications at midnight on the closing date.

Closing date: 30/01/2020

Salary: £41,526 to £51,034 per annum according to relevant experience.

Employment type: Fixed Term

Department: Biology, Medicine & Health

Hours per week: Full time

Contract Duration: From January 2020 until 31 December 2022 Job reference: BM&H-14959

Fluorophores that blink for STORM without any special buffer

Sounds good! No more buffer that stops working after one hour of imaging!

This company sells SaraFluor650 secondary antibodies. This fluorophore seems to be a natural blinker which does not need to reducing environment. Apparently it doesn’t need very bright lasers either!

Apparently they have also developed a green variant. So you can run 2-colour direct STORM on your favorite TIRF system! 😀

They also sell pH sensors and more so check their website and if you try, write a comment to let us know how things worked!

Microscopy facility job in Hungary

Dear Colleagues!

I would like to draw your attention to the open position at the Hungarian Center of Excellence for Molecular Medicine.

Best wishes,

Jozsef

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Advanced Core Facilities at HCEMM

High-quality molecular medicine-oriented research activities at the Hungarian Center of Excellence for Molecular Medicine (HCEMM) are supported by a matrix of advanced core facilities (ACFs). In the premises of the Hungarian Research Institutions, three ACFs are currently being set-up:

  • Functional Cell Biology and Immunology ACF (University of Szeged)
  • In vivo Imaging ACF (Semmelweis University, Budapest)
  • Single-Cell Omics ACF (Biological Research Center, Szeged)

The Call

The Project Steering Committee of the HCEMM project hereby announces a call for excellent, professional “Heads of Advanced Core Facilities”.

We are searching for Heads for each of the above ACFs who operate, manage, supervise, and develop the respective HCEMM AFC. We seek dynamic and interactive individuals with an excellent professional track record and demonstrated experience and interest in organizing and spearheading the ACF.

HCEMM is an inclusive, equal opportunity employer offering attractive working conditions and benefits appropriate to a molecular medicine research organization. Female candidates are strongly encouraged to apply.

An initial contract of 5 years will be offered to the successful candidates; this is foreseen to be extended to a maximum of 9 years, subject to an external, independent, international review. Successful candidates will receive an attractive remuneration package and an opportunity to recruit two technicians for each ACF. Importantly, the selected ACF Heads will be constantly helped and trained by heads and managers of the state-of-the-art system of Core Facilities at EMBL.

Technical notes

Please visit https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.hcemm.eu&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C096431407c66429c33c408d77ed8c1e5%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637117341763082188&sdata=AWRzMmDqkw%2B6Qtuctsvw1qmenkmrhiOUEoijTilFKFs%3D&reserved=0 for more details related to HCEMM (Synopsis of HCEMM), to the positions (Description of positions) and to the ACFs (Technical description of the ACFs) as well as the Evaluation process & Criteria.

Applicants should submit a cover letter, a CV, a detailed presentation of the professional experience, and a concise description of research interest and plans, all edited as a single PDF file, to career@hcemm.eu by January 17, 2020. Please also arrange for 3 letters of recommendation to be also emailed to career@hcemm.eu by the same deadline.

József Maléth M.D., Ph.D.

Principal Investigator, Research associate

MTA-SZTE Momentum Epithel Cell Signalling and Secretion Research Group University of Szeged, First Department of Medicine Koranyi alley 8-10, Szeged, H-6720, Hungary

Tel: +36-62-545-201

Cell: +36-70-4166500

E-mail: jozsefmaleth1@gmail.com;

maleth.jozsef@med.u-szeged.hu

Multiplexed immunostaining

It is not easy to find enough antibodies that work together to be able to label a sample with more than 4 antibodies at the same time. And even 4 is pushing it.

This paper describes a new immunostaining multiplexing method called 4i. The method is based on a special imaging buffer that prevents the antibody from being strongly bound to the sample due to the imaging process. This allows the authors to detach the antibody with gentle treatments, leaving the sample in a good shape and ready for another round of labeling and imaging.

Using this method they have successfully labelled the same sample with 40 different primary/secondary of the shelf antibody pairs!