If you are tired of DAPI bleeding through your weak green channel but are stuck to using blue for the nucleus, you might want to give a try to Syto41.
Must better excitation efficiency at 405 nm and much narrower emission spectrum!
And Syto also comes in other colors. 🙂
The next big thing in microscopy comes straight from the sky! Apparently astronomers have been using adaptive optics for years to improve their images and it is only getting into our microscopes now!!
Adaptive optics takes the nightmarish situation seen in b and puts it back straight as in a! This is done by measuring the wave front and deforming a mirror to reshape the wave front to perfection!
Sounds like a dream but I have actually seen it in action at the 2016 AQLM course and there was a definite WOW effect! 🙂
Check this review to learn more
Just imaging being able to image very tiny details in your sample on your favorite microscope without using super resolution!!
Expansion microscopy is definitely worth trying if your sample is fixed and what you want to measure is smaller than the detection limit of the microscope (around 250 nm)! There are people at Scilife who have implemented it and he is happy to collaborate! Just ask us! 🙂
This is just one example among many where expansion microscopy did wonders!