There are loads of multiwell plates and chamber slides on the market but it is hard to get any that fulfill these criteria:
- They do not kill the cells (unfortunately, this is not a joke!)
- They do not leak (no joke either! Sigh…)
- They have a low skirt: so they can be completely imaged (including outer part of outer wells) with our Nikon 20x air, NA 0.75, WD 1mm objectives. The ‘skirt’ is the rim at the edge of the plate. A high skirt raises the plate so that it becomes impossible to image the wells at the edges of the plate as the objective bumps into the insert edge.
- They have a #1.5 glass bottom
- They don’t cost a bomb.
We recently found a great source of plates and chambers that fulfill all these criteria and are very flat! Check the Zell Kontakt website (email under Legal disclaimer)! 🙂